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Ａ组轮状病毒中根据基因９的不同目前至少已发现有１３个不同Ｇ血清型，其中能引起人类致病的有Ｇ１－Ｇ４，Ｇ８，Ｇ９和Ｇ１２型。建立可靠的血清型鉴定技术对于轮状病毒疫苗的研制和分子流行病学的研究具有重要意义。本文首次报导了一种鉴定轮状病毒Ｇ血清型的新方法，利用已知有关轮状病毒ＶＰ７基因的序列资料，设计合成了一套鉴定轮状病毒Ｇ血清型的寡核苷酸探针，利用地高辛标记上述探针。待检品经反转录ＰＣＲ扩增后与上述一套寡核苷酸探针分别进行杂交得以确定其血清型。这一方法与目前常用的套式ＰＣＲ方法相比更适合于大量样品的操作而且结果可靠。用这一方法对本实验室组建的四株基因重配疫苗株进行实验，其结果与套式ＰＣＲ方法完全一致。
At present, at least 13 different G serotypes have been found in group A rotavirus according to the difference of gene 9, of which G1 to G4, G8, G9 and G12 types cause human pathogenicity. The establishment of reliable serological identification technology is of great significance for the research of rotavirus vaccine and molecular epidemiology. This is the first report of a new method for the identification of rotavirus G serotypes. Based on the known sequence information about the rotavirus VP7 gene, a set of oligonucleotide probes for the identification of rotavirus G serotypes was designed and synthesized. Digoxigenin was used to label the above probes. The products to be seized by reverse transcription PCR amplification and the above set of oligonucleotide probes were hybridized to determine the serotypes. This method is more suitable for the operation of a large number of samples than the currently used nested PCR method and the result is reliable. Using this method, four recombinant DNA vaccine strains established in our laboratory were tested and the results were in good agreement with the nested PCR method.