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目的:预测EB病毒LMP-1、BALF4及BARF1片段的B细胞表位。方法:采用Protean软件分析EBV的LMP1、BALF4及BARF1三个氨基酸片段的亲水性、表面可能性、抗原指数及其二级结构中的柔性区域,并结合吴玉章的抗原指数预测法预测其B细胞表位。结果:B细胞表位可能位于LMP-1 N端第356-358,2-19,249-314区段,BARF1N端第8-11,18-25,41-49,203-211区段,BALF4N端第385-387,833-845,398-415,427-435,453-458,23-32,466-473,234-250,642-652区段;另外LMP-1第185-223、BARF1第265-272,132-135以及BALF4第827-832区段内或附近也可能存在B细胞表位。结论:用多参数同时预测LMP-1、BALF4及BARF1的B细胞表位,为制备高效的鼻咽癌血清学诊断试剂和表位疫苗奠定了理论基础。
Objective: To predict the B cell epitopes of EBV LMP-1, BALF4 and BARF1 fragments. Methods: Proteins were used to analyze the hydrophilicity, surface potential, antigen index and the flexible regions in the secondary structure of three amino acid fragments of LMP1, BALF4 and BARF1 in EBV. The B cells were predicted by the antigenic index prediction method of Wu Yuzhang gauge. RESULTS: The B cell epitopes could be located in the 356th to 358th, 2-19th, 249th to 314th LRP-1 N-terminus, the 8th to 18th, the 18th to the 25th, the 41st to the 49th, the 203th to the 11th to the BARF1N, 387, 833-845, 398-415, 427-435, 453-458, 23-32, 466-473, 234-250, 642-652 sections; additionally, LMP-1 sections 185-223, BARF1 sections 265-272, 132-135 and BALF4 sections 827-832 B cell epitopes may be present. CONCLUSION: Simultaneous prediction of B cell epitopes of LMP-1, BALF4 and BARF1 by multiple parameters provides a theoretical basis for the preparation of an efficient serological diagnostic reagent and epitope vaccine for nasopharyngeal carcinoma.