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目的 研究人神经生长因子（ Ｈ Ｎ Ｇ Ｆ） 对大鼠中枢胆碱能神经元轴突切断后的保护作用。方法 向双侧海马伞切断所致的痴呆大鼠脑室内注入 Ｈ Ｎ Ｇ Ｆ， 采用穿梭箱训练， 检测大鼠学习记忆功能恢复状况， 对脑内隔斜带复合体（ Ｓ Ｄ Ｂ） 中隔区（ Ｍ Ｓ） 胆碱能神经元采用胆碱乙酰基转移酶（ Ｃ Ｈ Ａ Ｔ） 免疫组化法观察其存活状态。结果 Ｈ Ｎ Ｇ Ｆ 组大鼠术后两周内穿梭箱回避次数高于对照组（ Ｐ＜ ００１） ， 回避潜伏期短于对照组（ Ｐ＜ ００１） 。免疫组化及图像分析显示， 其胆碱能神经元存活状态优于对照组。结论 Ｈ Ｎ Ｇ Ｆ 对大鼠中枢胆碱能神经元损伤具有短期保护作用； 减缓轴突切断引起的退行性变， 对痴呆大鼠学习记忆功能恢复有促进作用。
Objective To study the protective effect of human nerve growth factor (H N G F) on the axons of central cholinergic neurons in rats. Methods HNGF was injected into the ventricle of rats with dementia induced by bilateral hippocampal cut off. The rats were trained by shuttle box to detect the recovery of learning and memory in rats. The cholinergic neurons in the septum (M s) were stained by choline acetyltransferase (C H A T) immunohistochemistry. Results The number of avoidance of shuttle box in HNGF group was higher than that in control group (P <001), and the avoidance latency was shorter in HNGF group than in control group (P <001). Immunohistochemistry and image analysis showed that cholinergic neurons survived better than the control group. Conclusion H N G F has a short-term protective effect on central cholinergic neuron injury in rats. Decreasing axonal degeneration caused by axonal desection can promote the recovery of learning and memory in dementia rats.