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以粳稻品种日本晴在组织培养过程中分离出来的一个半显性矮秆突变体Si-dd1为研究对象,通过形态学分析,发现与野生型日本晴相比,矮秆Si-dd1(AA)和半矮秆Si-dd1(Aa)植株的株高降低。结实率下降,生育期延长,一次枝梗和二次枝梗增加。激素处理结果表明突变体Si-dd1(AA)与野生型对油菜素内酯反应基本相同,而在高浓度赤霉素处理下,突变体Si-dd1(AA)表现为一定程度上的钝感。Western blot对GID2表达量分析也确定这一结果。组织切片实验表明,突变体Si-dd1(AA)相对于野生型叶片主脉气孔变小,叶肉细胞增多,茎维管束数目增加。遗传分析结果表明该突变体基因受一对核基因控制。进一步利用分子标记将该基因定位在水稻第6染色体约244kb区间内,目前该区段并未发现已报道的矮秆相关基因。
A semi-dominant dwarf mutant, Si-dd1, isolated from tissue culture of japonica rice, Nipponbare, was used as a study object. Morphological analysis showed that dwarf Si-dd1 (AA) Height of dwarf Si-dd1 (Aa) plants decreased. Seed setting rate decreased, growth period extended, primary branch and secondary branch increased. The hormonal treatment results showed that the mutant Si-dd1 (AA) reacted with wild-type to brassinolide approximately the same, whereas the mutant Si-dd1 (AA) showed a certain degree of insensitivity under high concentration of gibberellic acid . Western blot analysis of GID2 expression also confirmed this result. Tissue sectioning experiments showed that the stoma of the mutant Si-dd1 (AA) relative to the wild-type leaves became smaller, mesophyll cells increased, and the number of stem vascular bundles increased. Genetic analysis showed that the mutant gene was controlled by a pair of nuclear genes. The molecular marker was further used to locate the gene in the region of about 244kb on chromosome 6 of rice. At present, no dwarf related gene has been reported in this region.