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目的:检测TFIIB相关因子2(TFIIB-related factor 2,BRF2)基因在人食管鳞状细胞组织、癌旁组织及正常食管组织中的表达,分析BRF2在食管鳞状细胞癌发生、发展及预后中的意义。方法:选取2007年1月至2008年1月在山东大学齐鲁医院胸外科行手术治疗的食管鳞状细胞患者74例,应用RT-PCR和免疫组化方法检测食管鳞状细胞组织、癌旁组织和正常食管组织中BRF2 mRNA和蛋白表达水平。结果:食管鳞状细胞癌及其癌旁组织中BRF2 mRNA表达水平明显高于正常食管组织。食管鳞状细胞组织、癌旁组织和正常食管组织中BRF2蛋白表达阳性率分别为54.5%、32.5%和7.5%,癌组织、癌旁组织中BRF2蛋白的阳性率均显著高于正常食管组织(P<0.05)。随着食管鳞状细胞分化程度升高,BRF2蛋白阳性率显著下降,Ⅲ期和Ⅳ期食管鳞状细胞组织中BRF2蛋白阳性率明显高于Ⅰ期和Ⅱ期(72.7%,73.3%vs 35.7%,34.8%,P<0.05),且生存3年以下的食管鳞状细胞癌患者BRF2表达明显高于3年以上者(69.2%vs 38.2%,P<0.05),吸烟患者预后BRF2蛋白阳性率明显高于非吸烟患者(61.1%vs 30.4%,P<0.05)。结论:食管鳞状细胞癌组织高表达BRF2蛋白,BRF2 mRNA和蛋白与患者不良预后相关,可能作为食管鳞状细胞预后判断的参考指标之一。
OBJECTIVE: To detect the expression of TFIIB-related factor 2 (BRF2) gene in human esophageal squamous cell carcinoma tissues, adjacent normal tissues and normal esophageal tissues, and to analyze the expression of BRF2 in esophageal squamous cell carcinomas Meaning. Methods: From January 2007 to January 2008, 74 patients with esophageal squamous cell carcinoma underwent surgical treatment at Department of Thoracic Surgery, Qilu Hospital of Shandong University from January 2007 to January 2008. RT-PCR and immunohistochemistry were used to detect the expression of esophageal squamous cell carcinoma, And normal esophageal tissue BRF2 mRNA and protein expression levels. Results: The expression of BRF2 mRNA in esophageal squamous cell carcinoma and adjacent tissues was significantly higher than that in normal esophageal tissues. The positive rates of BRF2 protein in esophageal squamous cell carcinoma tissues, paracancerous tissues and normal esophageal tissues were 54.5%, 32.5% and 7.5% respectively. The positive rates of BRF2 protein in cancer tissues and paracancerous tissues were significantly higher than those in normal esophageal tissues P <0.05). With the esophageal squamous cell differentiation increased, the positive rate of BRF2 protein decreased significantly. The positive rate of BRF2 protein in stage Ⅲ and Ⅳ esophageal squamous cells was significantly higher than that in stage Ⅰ and Ⅱ (72.7%, 73.3% vs 35.7% , 34.8%, P <0.05). The expression of BRF2 protein in esophageal squamous cell carcinoma was significantly higher than that in patients with esophageal squamous cell carcinoma for more than 3 years (69.2% vs 38.2%, P <0.05) Higher than non-smoking patients (61.1% vs 30.4%, P <0.05). Conclusion: The high expression of BRF2 protein, BRF2 mRNA and protein in esophageal squamous cell carcinoma is associated with poor prognosis of patients with esophageal squamous cell carcinoma, which may be one of the reference indicators for predicting the prognosis of esophageal squamous cell carcinoma.