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目的:获取15种磺胺类药物(SAs)的ESI正离子模式下高分辨质谱碎片信息,并研究其裂解规律,探讨高能诱导裂解(HCD)技术的应用价值。方法:利用LTQ-Orbitrap XL线性离子阱-轨道离子阱高分辨组合式质谱仪的HCD技术对常见的15种SAs在高能裂解池中的裂解规律进行研究,并对比HCD和碰撞诱导解离(CID)技术两种裂解模式的差异。结果:15种SAs均产生共有碎片离子峰:[C6H6NSO2]+m/z156.01138、[C6H6NO]+m/z 108.04439、[C6H6N]+m/z 92.04948和[C5H5]+m/z 65.03858;与CID相比,HCD技术能提供更为丰富的裂解碎片信息,改善CID裂解中产生的低质量碎片丢失(cut-off)效应。结论:HCD技术能够提供丰富的裂解碎片信息,为磺胺类化合物的结构解析和确证提供更详实的依据。
OBJECTIVE: To obtain the information of fragmented high resolution MS spectra of 15 sulfonamides (SAs) under ESI positive ion mode and to study their cleavage patterns and to explore the value of high energy induced lysis (HCD). Methods: The cleavage regularities of 15 common SAs in high energy pyrolysis cell were studied by HCD technique using the LTQ-Orbitrap XL linear ion trap-orbit ion trap high resolution combined mass spectrometer. HCD and collision-induced dissociation (CID) Technical difference between the two modes of lysis. Results: All 15 SAs produced common fragment ion peaks: [C6H6NSO2] + m / z 156.01138, [C6H6NO] + m / z 108.04439, [C6H6N] + m / z 92.04948 and [C5H5] + m / z 65.03858; HCD technology provides richer fragment information than CID and improves the quality of cut-off caused by CID cleavage. Conclusion: The HCD technique can provide abundant fragments of pyrolytic fragments and provide a more detailed basis for structural elucidation and confirmation of sulfonamides.