论文部分内容阅读
目的:研究外源性生长激素(GH)和生长抑素(SS)对人结肠癌细胞株HT-29的影响,并探讨其作用机制。方法:人结肠癌细胞株HT-29分成正常对照组、生长抑素组(SS组)、生长激素组(GH组)和生长抑素+生长激素组(GH+SS组)。MTT法测定细胞抑制率,流式细胞仪测定细胞周期分布、增殖指数、凋亡率,RT-PCR方法测定bcl-2及baxmRNA水平。结果:生长抑素能够明显抑制人结肠癌细胞株HT-29增殖(P<0.01)、降低S期和G2/M期细胞比例(P<0.05)、降低增殖指数(PI)(P<0.05)、促进细胞凋亡(P<0.01)、降低bcl-2mRNA表达(P<0.05)、提高baxmRNA表达(P<0.01),生长激素则无明显作用。GH+SS组表现与SS组相似。结论:生长抑素可能通过抑制G0/G1期细胞进入S期和G2/M期以及促进细胞凋亡两种途径抑制体外培养的人结肠癌细胞株HT-29增殖。生长抑素可能是通过改变bax基因和bcl-2基因的表达影响肿瘤细胞的凋亡。生长激素对体外培养的人结肠癌细胞株HT-29无显著影响。
Objective: To study the effects of exogenous growth hormone (GH) and somatostatin (SS) on human colon cancer cell line HT-29 and to explore its mechanism of action. Methods: Human colon cancer cell line HT-29 was divided into normal control group, somatostatin group (SS group), growth hormone group (GH group) and somatostatin + growth hormone group (GH+SS group). The cell inhibition rate was determined by MTT assay. Cell cycle distribution, proliferation index and apoptosis rate were measured by flow cytometry. The levels of bcl-2 and bax mRNA were measured by RT-PCR. RESULTS: Somatostatin significantly inhibited the proliferation of human colon cancer cell line HT-29 (P<0.01), decreased the proportion of S phase and G2/M phase cells (P<0.05), and decreased the proliferation index (PI) (P<0.05). , Promote apoptosis (P <0.01), reduce bcl-2 mRNA expression (P <0.05), increase bax mRNA expression (P <0.01), growth hormone has no significant effect. The performance of the GH+SS group was similar to that of the SS group. CONCLUSION: Somatostatin may inhibit the proliferation of human colon cancer cell line HT-29 in vitro by inhibiting G0/G1 phase cells entering S phase and G2/M phase and promoting apoptosis. Somatostatin may affect the apoptosis of tumor cells by changing the expression of bax and bcl-2 genes. Growth hormone had no significant effect on human colon cancer cell line HT-29 cultured in vitro.