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Through observation about the restoration of male fertility of F2 and BC, progeny, we found that the restoring line Fengkel had two duplicating restorer genes. The restorer gene R/5 in Fengkel background was located on chromosome 5L by SSR method; it linked with bnlg1711, bnlg1346 and phi058, the genetic distances with bnlg1711, bnlg1346, and phi058 were 7.51cM, 1.68cM, and 9.87cM respectively; the restorer gene Rf4 was mapped on chromosome 8S linked with bnlg2307.
Through observation about the restoration of male fertility of F2 and BC, progeny, we found that the restoring line Fengkel had two duplicating restorer genes. The restorer gene R / 5 in Fengkel background was located on chromosome 5L by SSR method; it linked with bnlg1711 , bnlg1346 and phi058, the genetic distances with bnlg1711, bnlg1346, and phi058 were 7.51 cM, 1.68 cM, and 9.87 cM respectively; the restorer gene Rf4 was mapped on chromosome 8S linked with bnlg2307.