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目的观察兔急性房颤后心房肌基质金属蛋白酶-13(MMP-13)表达变化及厄贝沙坦对其的影响,探讨MMP-13和厄贝沙坦在房颤后心房重构中的作用。方法将2005年1月至6月哈尔滨医科大学附属第一临床医院动物中心实验室提供的60只兔随机分为2组:未给药组(ND,n=30)和给药组(D,n=30),给药组兔按厄贝沙坦30mg/(kg.d)灌胃3周;再随机分成3个亚组:对照组(1,n=10)、假手术组(2,n=10)、房颤8h组(3,n=10)。通过免疫组化法测定各组兔右房心肌中MMP-13、金属蛋白酶组织抑制因子-3(TIMP-3)、血管紧张素受体1(AT1)和血管紧张素受体2(AT2),通过放射免疫法测定右房心肌中血管紧张素Ⅱ(AngⅡ)。结果未给药房颤8h组右房心肌中MMP-13较对照组升高(P<0.05),TIMP-3无明显变化,AT1极显著升高(P<0.001),AT2无明显变化,AngⅡ极显著升高(P<0.001)。给药对照组AT1显著降低(P<0.01),MMP-13、TIMP-3、AT2和AngⅡ无显著变化;房颤8h组心房肌中MMP-13无显著升高(P>0.05),AT1极显著升高(P<0.001),但较未给药组降低(P<0.001),AT2无变化,AngⅡ极显著升高(P<0.001),与未给药组比较差异无显著性意义。结论急性房颤后心房肌中MMP-13表达升高,MMP-13在此后的心房结构重构中起重要作用。厄贝沙坦能抑制房颤后MMP-13升高,从而抑制了房颤后心房重构的进程。
Objective To investigate the changes of matrix metalloproteinase-13 (MMP-13) expression in atrial myocardium and the effect of irbesartan on acute myocardial fibrosis in rabbits and to explore the role of MMP-13 and irbesartan in atrial fibrillation after atrial fibrillation . Methods Sixty rabbits from the First Affiliated Hospital of Harbin Medical University Animal Center Laboratory from January 2005 to June 2005 were randomly divided into two groups: untreated group (ND, n = 30) and administration group (D, n = 30). The rabbits in the treatment group were treated with irbesartan 30mg / (kg.d) for 3 weeks. The rabbits were randomly divided into three subgroups: control group (n = 10), sham operation group (n = n = 10), atrial fibrillation 8h group (3, n = 10). The expressions of MMP-13, TIMP-3, AT1 and AT2 in right atrium of each group were determined by immunohistochemistry. Angiotensin Ⅱ (Ang Ⅱ) in right atrium myocardium was determined by radioimmunoassay. Results No significant changes of TIMP-3, AT1 (P <0.001), no significant change of AT2, AngⅡ Significantly increased (P <0.001). There was no significant difference in the expression of AT1 (P <0.01), MMP-13, TIMP-3, AT2 and AngⅡ in the control group, no significant increase in the atrial myocardium 8h after atrial fibrillation (P> 0.05) (P <0.001), but there was no change in AT2 and AngⅡ (P <0.001), but there was no significant difference compared with the untreated group (P <0.001). Conclusion The expression of MMP-13 in atrial myocardium after acute atrial fibrillation is increased, and MMP-13 plays an important role in the remodeling of atrial structure. Irbesartan can inhibit the increase of MMP-13 after atrial fibrillation, thus inhibiting the process of atrial fibrillation after atrial remodeling.