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本文采用不易通过血脑屏障的胆碱酯酶抑制剂──新斯的明(NS)和乙酰胆碱合成抑制剂──密胆碱(HC-3)经脑室、皮下及腹腔注射，分别升高和降低中枢及外周ACh的水平，以观测其对大鼠痛阈和间脑、尾核内甲硫氨酸脑啡肽(Met-ENK)含量的影响。结果发现：①新斯的明可明显提高大鼠的痛阈和脑内Met-ENK的含量；脑室给药的效应明显大于皮下给药者；②新斯的明有加强电针的镇痛效应，脑室给药对尾核内Met-ENK含量的作用明显；③密胆碱可明显抑制大鼠的痛阈，降低脑内Met-ENK的水平，脑室给药的效应作用更广泛；④电针可对抗密胆碱对痛阈和脑内Met-ENK的抑制作用，其中对腹腔给药者痛阈影响较大，对脑室给药者Met-ENK含量影响较大。
In this study, cholinesterase inhibitors, NS and acetylcholine synthesis inhibitors, not easy to cross the blood-brain barrier, were injected intracerebroventricularly Reduce central and peripheral ACh levels in order to observe its impact on pain threshold and methionine enkephalin (Met-ENK) content in the diencephary and caudate nucleus. The results showed that: (1) Neostigmine could significantly increase the pain threshold and the level of Met-ENK in the brain; the ventricular administration effect was significantly greater than that in the subcutaneous administration; (2) Neostigmine had an analgesic effect , Intracerebroventricular injection of Met-ENK content in the caudate nucleus significantly; ③ acholine can significantly inhibit the pain threshold in rats, reduce the level of Met-ENK in the brain, intraventricular administration of the role of a wider range; It can antagonize the inhibition of the pain threshold and Met-ENK in the brain, which has a greater impact on the pain threshold of intraperitoneal drug delivery and greater impact on the content of Met-ENK in intraventricular administration.