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目的观察上皮间质转化(EMT)标记物在人胚胎肝胆管板发育过程中的表达,探讨胆管板发育过程中EMT的发生和意义。方法应用免疫组化法观察EMT相关标记物细胞角蛋白19(CK19)、波形蛋白(vimentin)、α-平滑肌肌动蛋白(α-SMA)在31例8~40周龄人胚胎肝胆管板发育过程中的表达和变化情况。结果从8周龄开始,随着胆管板阶段、胆管板重新塑形阶段、胆管形成阶段的发展,汇管区内CK19染色阳性细胞百分率也逐渐增加,而vimentin和α-SMA阳性细胞百分率则逐渐减少,CK19染色阳性细胞百分率分别与vimentin和α-SMA阳性细胞百分率呈负相关(CK19与vimentin:r=-0.820,P<0.001;CK19与α-SMA:r=-0.797,P<0.001)。9周龄胚胎肝胆管板细胞开始表达vimentin,13~19周时达到高峰,之后减少,28周时检测不到;14~32周龄胚胎肝部分发育中的胆管细胞也表达vimentin,观察到汇管区vimentin阳性的肌纤维母细胞(pMFs)向胆管(板)内整合、移行。通过连续切片观察到9~32周龄胚胎肝部分胆管板或胆管细胞共表达vimentin和CK19。结论人胚胎肝胆管板发育过程中存在间质上皮转化,与胆管形成有关。
Objective To observe the expression of epithelial-mesenchymal transition (EMT) markers during the development of human embryonic hepatobiliary board and to explore the occurrence and significance of EMT during the development of biliary duct. Methods Immunohistochemistry was used to detect the expression of EMT-related markers cytokeratin 19 (CK19), vimentin and α-smooth muscle actin (α-SMA) in 31 patients with embryonic development of hepatobiliary duct from 8 to 40 weeks The process of expression and changes. Results From 8 weeks of age, the percentage of CK19-positive cells in the portal area gradually increased, while the percentage of vimentin and α-SMA positive cells decreased with the development of the bile duct plate, bile duct plate remodeling and bile duct formation. , CK19 staining positive cells were negatively correlated with the percentage of vimentin and α-SMA positive cells (CK19 and vimentin: r = -0.820, P <0.001; CK19 and α-SMA: r = -0.797, P <0.001). The expression of vimentin began to occur in 9-week-old embryonic hepatobiliary plate cells, and peaked at 13 to 19 weeks, then decreased and was undetectable at 28 weeks. Vimentin was also expressed in the developing part of the fetal liver from 14 to 32 weeks of age, Area vimentin-positive myofibroblasts (pMFs) to the bile duct (plate) integration, migration. By continuous sectioning, vimentin and CK19 were co-expressed in the bile duct or cholangiocytes from 9 to 32-week-old embryonic liver. Conclusion There is interstitial epithelial transformation in the development of human embryonic hepatobiliary ductal plate, which is related to the formation of bile ducts.