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目的：探讨IL-8和INF-α在RV肠炎发病中的作用及临床意义。方法：采用双抗体夹心ELISA法。结果：RV肠炎患儿急性期大便IL-8和TNF-α水平与对照组相比显著升高（P＜0．01），恢复期二者迅速下降，但仍高于对照组（P＜0．05）。急性期中重症组大便IL-8和TNF-α水平显著高于轻症组（P＜0．01），且大便IL-8水平和TNF-α水平呈显著正相关（P＜0．01）。结论：IL-8和TNF-α作为重要的炎症介质共同参与了RV肠炎肠道的炎症反应，二者的大便水平与RV肠炎病情轻重密切相关。
Objective: To investigate the role and clinical significance of IL-8 and INF-α in the pathogenesis of RV enteritis. Methods: Double antibody sandwich ELISA was used. Results: The levels of IL-8 and TNF-α in stool of children with RV enteritis were significantly higher than those in the control group (P <0.01), while those in the recovery period were both decreased rapidly (P <0) .05). The levels of stool IL-8 and TNF-α in the acute and moderate-severe group were significantly higher than those in the mild group (P <0.01), and there was a significant positive correlation between stool IL-8 and TNF-α (P <0.01). CONCLUSION: IL-8 and TNF-α are involved in the intestinal inflammatory response of RV enteritis as an important inflammatory mediator. The stool level of both is closely related to the severity of RV enteritis.