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目的:观察自身免疫性心肌病(AIC)小鼠模型和病毒性心脏病(VHD)患者血清抗心肌线粒体腺嘌 呤核苷异位酶(ANT)抗体的演变规律。方法:建立ANT合成肽AIC小鼠模型,ELISA法定期监测小鼠血清抗 ANT抗体,观察心肌病理变化;对不同病程VHD患者血清进行抗ANT抗体检测。结果:AIC小鼠模型抗ANT 抗体阳性率随着病程延长,呈先上升后下降趋势,病理检查结果提示心肌组织有慢性炎症的改变。在不同病程 VHD患者中,血清抗ANT抗体阳性率也呈现先升高后降低的变化,临床随访发现12.9%病毒性心肌炎转化为 扩张型心肌病。AIC小鼠模型与不同病程VHD患者血清抗ANT抗体演变具有一致性(r=0.53,P>0.05)。 结论:抗ANT抗体水平的变化与心肌病进程相关。
Objective: To observe the evolution of antimyocardial mitochondrial adenine nucleotide enzyme (ANT) antibody in patients with autoimmune cardiomyopathy (AIC) mouse model and viral heart disease (VHD). Methods: The ANT synthetic peptide AIC mouse model was established. The serum anti-ANT antibody was monitored by ELISA. The pathological changes of myocardium were observed. Anti-ANT antibody was detected in sera of patients with different course of VHD. Results: The positive rate of anti-ANT antibody in AIC mouse model increased at first and then decreased with the prolongation of its duration. The pathological examination showed that the myocardial tissue had chronic inflammatory changes. In patients with different course of VHD, the positive rate of serum anti-ANT antibody also increased at first and then decreased. Clinical follow-up found that 12.9% of viral myocarditis was converted to dilated cardiomyopathy. The AIC mouse model was consistent with the development of serum anti-ANT antibody in patients with different course of VHD (r = 0.53, P> 0.05). Conclusion: The change of anti-ANT antibody level is related to the progression of cardiomyopathy.