论文部分内容阅读
目的:观察薯蓣皂苷元对甲状腺功能亢进大鼠肝功能和肝脏氧化应激状态的影响。方法:腹腔注射T4(0.5mg/kg)制备甲状腺功能亢进性肝损害大鼠模型,并分别给予60mg/kg和30mg/kg剂量薯蓣皂苷元进行干预。4周后取血检测肝功能、血清总抗氧化能力、脂质过氧化产物TBARS含量,取肝脏检测TBARS、超氧化物歧物酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)、谷胱甘肽-S-转移酶(GST)活性,以及T-GSH/GSSG比值。结果:腹腔注射T4可引起大鼠肝功能损害,表现为ALT、AST、ALP水平显著升高。薯蓣皂苷元治疗4周可明显改善甲亢大鼠肝功能,降低ALT、AST、ALP水平。与正常对照组比较,甲亢大鼠血清总抗氧化能力明显降低,血和肝中TBARS水平均较正常对照组明显升高,而肝脏抗氧化酶SOD、GSH-Px、GR、GST活性和TGSH/GSSG比值均显著降低。治疗4周后,60mg/kg和30mg/kg剂量薯蓣皂苷元可使甲亢大鼠总抗氧化能力分别升高1.55倍和1.38倍,血和肝中TBARS水平分别下降42%和36%,21%和26%,同时肝脏抗氧化酶活性和T-GSH/GSSG比值均显著升高。结论:薯蓣皂苷元可通过多环节对抗氧化应激反应,对甲亢性肝损害发挥保护作用。
Objective: To observe the effects of diosgenin on liver function and hepatic oxidative stress in hyperthyroidism rats. Methods: Hyperthyroid rats were induced by intraperitoneal injection of T4 (0.5 mg / kg), and 60 and 30 mg / kg doses of diosgenin were administered respectively. Four weeks later, the blood was taken to measure the liver function, the total serum antioxidant capacity, the content of lipid peroxidation product TBARS, the content of TBARS, SOD, GSH-Px ), Glutathione reductase (GR), glutathione-S-transferase (GST) activity, and T-GSH / GSSG ratio. Results: Intraperitoneal injection of T4 can cause hepatic dysfunction in rats, manifested as significantly increased levels of ALT, AST and ALP. Diosgenin treatment for 4 weeks can significantly improve liver function in hyperthyroid rats, reduce ALT, AST, ALP levels. Compared with the normal control group, the serum total antioxidant capacity of hyperthyroidism rats decreased significantly, the levels of TBARS in blood and liver were significantly higher than those in the normal control group, while the activity of SOD, GSH-Px, GR, GST and TGSH / GSSG ratio were significantly lower. After 4 weeks of treatment, diosgenin at doses of 60 mg / kg and 30 mg / kg increased the total antioxidant capacity of hyperthyroid rats by 1.55 times and 1.38 times, respectively, and the levels of TBARS in blood and liver decreased by 42% and 36%, 21% And 26%, respectively, while liver antioxidant enzyme activity and T-GSH / GSSG ratio were significantly increased. Conclusion: Diosgenin can protect against hyperthyroid liver injury through multiple links to anti-oxidative stress response.