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建立构象差异凝胶电泳(conformation difference gel electrophoresis,CDGE)检测亚甲基四氢叶酸还原酶(methylenetetrahydrofolate reductase,MTHFR)基因A1298C多态性的方法。分别用PCR-CDGE和PCR-RFLP对10例样本的MTHFR基因A1298C多态性进行分型。CDGE结果显示有8例样本表现为迁移率慢的1条带,1例样本表现为迁移率快的1条带,1例样本表现为2条带。酶切结果显示AA基因型有8例,AC基因型和CC基因型各1例。CDGE和酶切结果的对比显示二者的结果是一致的。CDGE是一种可以对MTHFR基因A1298C多态性分型的方法。
To establish a method for detecting polymorphism of methylenetetrahydrofolate reductase (MTHFR) gene A1298C by conformation difference gel electrophoresis (CDGE). PCR-CDGE and PCR-RFLP were used to classify the MTHFR gene A1298C polymorphism in 10 samples. The results of CDGE showed that eight samples showed one band with slow mobility, one case showed one band with fast mobility and one case showed two bands. Results of enzyme digestion showed that there were 8 AA genotypes and 1 AC genotype and 1 CC genotype. A comparison of CDGE and digestion results shows that the two are consistent. CDGE is a method that can classify the A1298C polymorphism of MTHFR gene.