论文部分内容阅读
作者通过Southern印迹杂交技术,检测了87例急性、慢性白血病和骨髓增生异常综合征(MDS)病人的免疫球蛋白(Ig)和T细胞受体(TCR)基因排列方式,并结合形态学和免疫表型加以分析。87例病人中,急性淋巴细胞白血病(ALL)26例,慢性淋巴细胞白血病(CLL)10例,急性髓细胞白血病(AML)22例,MDS10例,慢粒(CML,均为Ph′+)急变19例。治疗前根据外周血和骨髓涂片检查确诊,形态学按FAB标准分类。并作细胞化学和免疫表型检查。利用Southern印迹杂交技术,对外周血和骨髓标本进行DNA检测。所用探针:Ig重链结合区(JH)、Igκ轻链恒区(Cκ)、Igλ轻链恒区(C_λ)和TCRβ链恒区。
The authors examined the arrangement of immunoglobulin (Ig) and T cell receptor (TCR) genes in 87 patients with acute, chronic leukemia and myelodysplastic syndrome (MDS) by Southern blot hybridization and combined morphological and immunological Phenotype to be analyzed. Of the 87 patients, 26 were acute lymphoblastic leukemia (ALL), 10 were chronic lymphocytic leukemia (CLL), 22 were acute myeloid leukemia (AML), 10 were MDS, and CML (both Ph ’+) 19 cases. According to the examination of peripheral blood and bone marrow smear before treatment, morphology was classified according to FAB standard. And cytochemistry and immunophenotype examination. Southern blotting was used to detect DNA in peripheral blood and bone marrow samples. The probes used were Ig heavy chain binding region (JH), Ig kappa light chain constant region (C kappa), Ig lambda constant region (C_ [lambda]) and TCR beta chain constant region.