论文部分内容阅读
Objective: To study the effect of oxymatrine on the expression of nuclear factor-kappa B(NF-κB) of Human benign epidermal keratinocytes line(HaCaT cells). Methods:HaCaT cells were cultured with different concentration of Oxymatrine(10μg/ml, 50μg/ ml and 100 μg/ml) for 24 h, then 10- mol/L Substance P was added to the cells. After 30 min, NF-κB expression in the cells was 5 observed by immunocytochemistry, NF-κB P65 protein expression was evaluated by Western blot, and the mRNA expression of NF-κB P65 was evaluated by reverse transcription polymerase chain reaction(RT-PCR). The 10- mol/L Substance P and culture medium were 5 added to the Substance P group and control group, respectively. Results:In control group, expression rate of positive cells, the expressions of protein and mRNA of NF-κB were all low. In Substance P group, when 10- mol/L Substance P was added, the expres-sions were all 5 increased(P < 0.05). But in Oxymatrine groups, the expression rate of positive cells, the expressions of protein and mRNA were all descended in a concentration-dependent manner(P < 0.05 or P < 0.01). Conclusion:Oxymatrine can down-regulate the expression of NF-κB of the HaCaT cells and may play an important role in regulating anti-inflammation and immunity.
Objective: To study the effect of oxymatrine on the expression of nuclear factor-kappa B (NF-κB) of human benign epidermal keratinocytes line (HaCaT cells). Methods:HaCaT cells were cultured with different concentration of Oxymatrine (10μg/ml, 50μg / ml and 100 μg/ml) for 24 h, then 10- mol/L Substance P was added to the cells. After 30 min, NF-κB expression in the cells was 5 observed by immunocytochemistry, NF-κB P65 protein expression was The study was evaluated by Western blot, and the mRNA expression of NF-κB P65 was evaluated by reverse transcription polymerase chain reaction (RT-PCR). The 10-mol/L Substance P and culture medium were 5 added to the Substance P group and control group. , respectively. Results: In control group, expression rate of positive cells, the expressions of protein and mRNA of NF-κB were all low. In Substance P group, when 10- mol/L Substance P was added, the expres-sions were All 5 increased (P < 0.05). But in Oxymatrine groups, the expression rate of positive cells, th E expressions of protein and mRNA were all descended in a concentration-dependent manner (P < 0.05 or P < 0.01). Conclusion: Oxymatrine can down-regulate the expression of NF-κB of the HaCaT cells and may play an important role in regulating Anti-inflammation and immunity.