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目的 :探讨血管内皮生长因子 (vascularendotherialgrowthfactor ,VEGF)在乳腺癌中的表达及其与乳腺癌P5 3蛋白在乳腺癌中的表达之间的关系 ,以及它们与乳腺癌临床病理特征间的关系。方法 :采用免疫组织化学方法检测 88例乳腺癌 ,10例正常乳腺组织以及 10例乳腺良性肿瘤手术标本的VEGF和P5 3蛋白的表达。结果 :在 88例乳腺癌病例中 ,有淋巴结转移的VEGF阳性表达率 (94.2 9% )明显高于无淋巴结转移的VEGF阳性表达率 (49.0 6 % ) (P <0 .0 0 1) ,VEGF阳性表达率与乳腺癌肿瘤大小无关系 (P >0 .1)。P5 3蛋白表达与乳腺癌淋巴结状况 (P >0 .5 )及肿瘤大小 (P >0 .1)无关系。在VEGF表达阳性患者的P5 3蛋白表达阳性率 (45 .76 % )明显高于VEGF表达阴性患者的P5 3蛋白表达阳性率 17.2 4% (P <0 .0 1)。结论 :VEGF在乳腺癌的发生发展及转移中发挥重要作用 ;P5 3基因失活和突变能降低血管生成阻止因子的分泌从而激发VEGF在肿瘤中的表达 ,促使乳腺癌的发生发展和转移。
OBJECTIVE: To investigate the relationship between the expression of vascular endothelial growth factor (VEGF) in breast cancer and the expression of P53 protein in breast cancer and their relationship with the clinicopathological features of breast cancer. Methods: The expressions of VEGF and P53 protein in 88 cases of breast cancer, 10 cases of normal breast tissue and 10 cases of benign breast tumor were examined by immunohistochemistry. Results: In 88 cases of breast cancer, the positive rate of VEGF with lymph node metastasis (94.2 9%) was significantly higher than that without lymph node metastasis (49.0 6%) (P0.01) The positive expression rate had no relation with the size of breast cancer (P> 0. 1). The expression of P53 protein had no correlation with lymph node status (P> 0.05) and tumor size (P> 0. 1) in breast cancer. The positive rate of P53 protein expression in patients with positive VEGF expression (45.76%) was significantly higher than that in patients with negative VEGF expression protein (17.2%, P <0.01). Conclusion: VEGF plays an important role in the development and metastasis of breast cancer. The inactivation and mutation of P5 3 gene can reduce the secretion of angiogenesis and induce the expression of VEGF in the tumor and promote the development and metastasis of breast cancer.