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目的:通过对染氟大鼠和小鼠氟斑牙的观察,进一步确定氟斑牙作为慢性氟中毒的诊断指标的重要性。方法:通过不同浓度和不同时间的氟染毒,利用数码体视显微镜观察Wistar大鼠和C57BL/6J小鼠氟斑牙的形态变化。将Wistar大鼠随机分为4组,每组8只,共计32只,分笼饲养,C57BL/6J小鼠4组,每组12只,共计48只,分8笼饲养。对照组:蒸馏水组,实验组三组:氟化钠分别为50 mg/L组,100 mg/L组和150 mg/L组。在染氟至6个月时,应用在体视显微镜观察大鼠和小鼠牙齿的动态改变。结果:染氟组Wistar大鼠和C57BL/6J小鼠牙齿均出现规则的斑纹、白垩状、延迟断裂、缺损等症状。随着染氟时间的延长,C57BL/6J小鼠氟斑牙的损伤程度大于Wistar大鼠。在慢性氟中毒大鼠和小鼠的牙齿变化中发现,大鼠和小鼠的牙齿变化程度与种属有着密切的关系。结论:通过利用体视显微镜对Wistar大鼠和C57BL/6J小鼠染氟6个月进行氟斑牙的观察分析,为慢性氟中毒鼠模型提供氟斑牙的形态学的详实依据。并且,可根据研究需要适宜选择氟斑牙的动物模型。
OBJECTIVE: To further confirm the importance of dental fluorosis as a diagnostic indicator of chronic fluorosis through the observation of fluorosis rats and mice dental fluorosis. Methods: The morphological changes of dental fluorosis in Wistar rats and C57BL / 6J mice were observed by digital stereo microscope with fluorine exposure at different concentrations and different times. The Wistar rats were randomly divided into 4 groups with 8 rats in each group. A total of 32 rats were housed in a cage. C57BL / 6J mice were divided into 4 groups (n = 12). A total of 48 rats were housed in 8 cages. Control group: distilled water group, experimental group three groups: sodium fluoride were 50 mg / L group, 100 mg / L group and 150 mg / L group. In stained fluorine to 6 months, the application of stereomicroscope in rats and mice to observe the dynamic changes in the teeth. Results: The teeth of Wistar rats and C57BL / 6J mice exposed to fluoride were regularly streaked, chalked, delayed ruptured and damaged. With the extension of fluoridation time, the degree of dental fluorosis in C57BL / 6J mice was greater than that in Wistar rats. Tooth changes in rats and mice with chronic fluorosis have found that the degree of tooth changes in rats and mice is closely related to species. CONCLUSION: The dental fluorosis model in rats with chronic fluorosis was provided with detailed and detailed evidence of the morphology of dental fluorosis by observing the dental fluorosis by fluoroscopy for 6 months in Wistar rats and C57BL / 6J mice using stereomicroscope. In addition, the animal model of dental fluorosis can be appropriately selected according to the research needs.