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目的探讨职业性锰接触工人DAN损伤修复基因X射线修复交叉互补基因1(XRCC1)C26304T位点和G27466A位点的多态性与神经行为改变的关系。方法选取工龄为≥0.5a的职业性锰接触冶炼工人200名和劳动强度相似不接触锰的工人94名,按照累积暴露指数将调查对象分为高、低接触组和对照组。利用垂直沟槽稳定试验和插板试验进行神经行为改变的检测;提取血白细胞DNA,利用PCR扩增限制性酶切法(PCR-RFLP)检测XRCC1C26304T位点和G27466A位点的单核苷酸多态性(SNPs)。结果高、低接触组中携带C26304T位点变异基因型TT的工人在垂直沟槽稳定试验的划痕长度、集合试验的得分均低于携带野生基因型CC和杂合基因型CT的工人,碰撞次数高于野生基因型和杂合基因型;对照组基因型之间差异无统计学意义(P>0.05)。高、低接触组中携带G27466A位点变异基因型AA的工人在垂直沟槽稳定试验的划痕长度、集合试验的得分均低于携带野生基因型GG和杂合基因型GA的工人,碰撞次数高于野生基因型和杂合基因型;对照组基因型之间差异无统计学意义(P>0.05)。结论携带XRCC1C26304T和G27466A位点变异基因型的工人是锰损伤的易感者;XRCC1基因位点C26304T和G27466A突变导致的多态性有可能作为检测锰致神经行为改变以及锰中毒风险评估的可用指标。
Objective To investigate the relationship between polymorphism and neurobehavioral changes of C26304T and G27466A loci in X-ray repair cross-complementary gene 1 (XRCC1) of occupational manganese exposed workers. Methods A total of 200 occupational manganese contact smelters with working age≥0.5a and 94 workers with similar working intensity without manganese exposure were selected and divided into high and low exposure groups and control groups according to cumulative exposure index. Vertical channel stability test and plug-board test were used to detect neurobehavioral changes. DNA of white blood cells was extracted. Single nucleotide polymorphisms of XRCC1C26304T site and G27466A site were detected by PCR-RFLP State (SNPs). Results The length of scratches in the vertical groove stability test and the scores of the set test in workers with high or low exposure group carrying TT variant genotype C26304T were lower than those carrying wild genotype CC and heterozygous genotype CT, The number of genotypes was higher than wild genotypes and heterozygous genotypes. There was no significant difference in genotypes between control groups (P> 0.05). In the high and low contact groups, the length of scratches in the vertical groove stability test for workers carrying the G27466A site variant genotype AA were lower than those in the group carrying the wild genotype GG and the heterozygous genotype GA, the number of collisions Higher than wild genotypes and heterozygous genotypes; there was no significant difference in genotypes of control group (P> 0.05). CONCLUSIONS: Workers with variant genotypes XRCC1C26304T and G27466A are susceptible to manganese injury. Polymorphisms caused by mutations in C26304T and G27466A at XRCC1 locus may be useful indicators for the assessment of behavioral changes in manganese-induced neurotoxicity and manganese risk assessment .