论文部分内容阅读
目的观察α-硫辛酸对高糖诱导的血管内皮细胞凋亡是否有保护作用。方法以不同浓度α-硫辛酸干预高糖作用下的血管内皮细胞(ECV304),共同孵育72h,以Annexin-FITC/PI双染流式细胞术和TUNEL法检测细胞凋亡。结果高糖明显增加内皮细胞凋亡(P<0.01);以不同浓度α-硫辛酸干预,内皮细胞凋亡明显减少(P<0.01),这种作用具有浓度依赖性。结论α-硫辛酸对高糖诱导的血管内皮细胞凋亡有保护作用。
Objective To observe whether α-lipoic acid has a protective effect on the apoptosis of vascular endothelial cells induced by high glucose. Methods The vascular endothelial cells (ECV304) were treated with different concentrations of α-lipoic acid under high glucose for 72 hours. Cell apoptosis was detected by Annexin-FITC / PI double staining and TUNEL. Results High glucose significantly increased the apoptosis of endothelial cells (P <0.01). With different concentrations of α-lipoic acid, the apoptosis of endothelial cells was significantly decreased (P <0.01), and this effect was concentration-dependent. Conclusion α-lipoic acid has a protective effect on the apoptosis of vascular endothelial cells induced by high glucose.