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目的探索不同的DNA疫苗初免-重组痘苗病毒加强免疫策略的效果,为建立最佳免疫方案提供依据。方法对小鼠进行DNA疫苗初免,之后分别加强免疫一针、两针和三针重组痘苗病毒VTT△C8-K3-gag,在不同时间点采取血样及制备脾细胞,检测针对痘苗病毒和HIV特异性体液免疫及细胞免疫应答。结果三种免疫策略中,DNA疫苗初免-VTT△C8-K3-gag加强免疫两次的效果最佳,诱导的针对p55的结合抗体滴度达到106,分泌IFN-γ的T细胞数为342/106细胞。针对痘苗病毒的体液和细胞免疫应答显著低于加强免疫三针诱导的免疫反应。结论DNA疫苗初免-VTT△C8-K3-gag加强免疫两针可诱导较高水平的HIV免疫应答,同时保证了较低水平的载体免疫反应。
Objective To explore the effect of different DNA vaccine prime-recombinant vaccinia vaccines in boosting immunization strategies and provide the basis for establishing optimal immunization programs. Methods After immunization of mice, DNA vaccines were immunized with one, two and three-dose recombinant vaccinia virus VTT △ C8-K3-gag respectively. Blood samples and splenocytes were prepared at different time points to detect vaccinia virus and HIV Specific humoral and cellular immune responses. Results Among the three immunization strategies, the DNA vaccine prime-VTT △ C8-K3-gag boosted twice with the best effect, the induced antibody titer against p55 was 106, and the number of IFN-γ-secreting T cells was 342 / 106 cells. The humoral and cellular immune responses to vaccinia virus were significantly lower than those of the three-needle-boosted immune response. Conclusion DNA vaccine prime-VTT △ C8-K3-gag booster immunization can induce higher levels of HIV immune response, while ensuring a lower level of carrier immune response.