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目的 探讨阿司匹林对人肝癌生长的影响及是否能诱导其凋亡。方法 用3H-TdR掺入、形态学观察、DNA末端原位标记法（Tunel）和流式细胞术等方法研究阿司匹林对人肝癌细胞株SMMC-7721生长及诱导凋亡的作用;建立人肝癌裸鼠原位移植瘤模型,观察阿司匹林对肝癌移植瘤生长的影响。结果 阿司匹林能显著抑制人肝癌细胞株SMMC-7721生长,当其浓度在1×10-1×10-7mol/L时,肝癌细胞3H-TdR掺入与浓度增加呈显著负相关（r=-0.918,P<0.01）。经1×10-3mol/L阿司匹林作用后可见较多肝癌细胞呈典型的细胞凋亡形态学变化,凋亡指数为（8.90±1.32）％,对照组为（0.50±0.35）％,两组比较,差异有显著性,流式细胞术也检测到其G1期前有一典型的亚二倍体凋亡峰,凋亡率为12.79％。阿司匹林能显著抑制人肝癌裸鼠原位移植瘤生长,抑瘤率为71.62％,实验中裸鼠存活率为100％,未见消化道出血、溃疡等不良反应。结论 阿司匹林能显著抑制人肝癌生长并能诱导其凋亡。
Objective To investigate the effect of aspirin on human hepatocellular carcinoma (HCC) growth and whether it can induce apoptosis. Methods The effects of aspirin on the growth and apoptosis of human hepatocellular carcinoma cell line SMMC-7721 were studied by 3H-TdR incorporation, morphological observation, Tunel and flow cytometry. In situ xenograft model of mice was used to observe the effect of aspirin on the growth of hepatocellular carcinoma. Results Aspirin significantly inhibited the growth of human hepatocellular carcinoma cell line SMMC-7721. When its concentration was 1 × 10-1 × 10-7 mol / L, 3H-TdR incorporation was negatively correlated with the concentration of aspirin (r = -0.918 , P <0.01). After 1 × 10-3mol / L aspirin treatment, more hepatocellular carcinoma cells showed the typical morphological changes of apoptosis, the apoptotic index was (8.90 ± 1.32)% in the control group and (0.50 ± 0.35)% in the control group , The difference was significant. Flow cytometry also detected a typical sub-diploid apoptotic peak before G1 phase, with a apoptotic rate of 12.79%. Aspirin can significantly inhibit the growth of orthotopic transplanted human hepatocellular carcinoma in nude mice, with a tumor inhibition rate of 71.62%. The survival rate of nude mice was 100%. No adverse reactions such as gastrointestinal bleeding and ulcer occurred. Conclusion Aspirin can significantly inhibit the growth of human hepatocellular carcinoma and induce its apoptosis.