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应用恒河猴做成亚慢性三硝基甲苯染毒（ＴＮＴ）模型，在染毒前，染毒中期和试验表明咖啡因在动物体内的清除呈一级动力学，半衰期为８。８８小时，这一结果说明咖啡因符合肝脏代谢模型药物的要求。ＴＮＴ染毒后咖啡因清除率（ＣＬｃａ）下降，而同期血清甘胆醚（ＣＡ）浓度无变化。这一结果说明ＣＬＣａ可能比ＣＡ能更早地反映接触ＴＮＴ后的机体反应，染毒三个月后处死动物直接测定肝混合功能氧化酶（ＭＦＯ）活性（Ｐ４５０及氨基比林脱甲基酶），发现咖啡因清除率和ＭＦＯ有很好的相半性。由于ＭＦＯ的变化往往是ＴＮＴ接触后机体的早期的反映，因此这一结果为使用ＣＬｃａ作为ＴＮＴ接触者的早期健康监护指标展示了一定的前景。
Application of rhesus monkeys made of sub-chronic trinitrotoluene (TNT) model, before the exposure, the mid-exposure and the test showed that the clearance of caffeine in animals was first-order kinetics, half-life of 8.88 hours, This result shows that caffeine meets the requirements of liver metabolism model drugs. After TNT exposure, the caffeine clearance rate (CLca) decreased while the serum CA level did not change during the same period. This result suggests that CLCa may reflect the response of the organism after exposure to TNT earlier than CA. Animals were sacrificed three months after exposure to directly determine the activity of liver mixed-function oxidase (MFO) (P450 and aminopyrine demethylase) , Found that caffeine clearance and MFO have a good half-life. Since changes in MFO tend to be early manifestations of the organism after TNT exposure, this result shows some promise for the use of CLca as an early health indicator for TNT contacts.