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[目的]本文评价膳食调查大豆异黄酮摄入量估算值与尿液中大豆异黄酮代谢物测定值之间的相关性。[方法]采用大豆食物频数问卷(SoyFFQ),对南京市不同社区共469名妇女大豆相关食品摄入量进行调查,利用美国食物成分数据,计算两种大豆异黄酮(染料木黄酮genistein和大豆苷元daidzein)每日摄入量;其中30名调查对象提供尿液样本,采用超高速液相色谱-串联质谱(UPLC-MS/MS)方法测定尿液中大豆异黄酮代谢物的浓度;采用高效液相(HPLC)方法测定部分大豆食品中染料木黄酮(genistein)和大豆苷元(daidzein)的含量。[结果]469名妇女膳食调查摄入量估算值:染料木黄酮(genistein):(50.7±31.6)mg/d,大豆苷元(daidzein):(33.4±35.9)mg/d;30份尿液中大豆异黄酮代谢物测定值:染料木黄酮(genistein):(515.9±674.5)μmol/mol,大豆苷元daidzein:(927.7±1107.2)μmol/mol。染料木黄酮(genistein)和大豆苷元(daidzein)膳食调查摄入量估算值与尿液中大豆异黄酮代谢物测定值相关系数分别为:0.59和0.57(P﹤0.05)。[结论]可以认为染料木黄酮(genistein)和大豆苷元(daidzein)摄入量估算值与尿液测定值具有相关性,为国内进行大豆异黄酮与乳腺癌发生风险的相关研究提供的可靠数据和方法。
[Objective] This study evaluates the correlation between dietary estimates of soy isoflavone intake and those of urine soy isoflavone metabolites. [Method] The soybean food frequency questionnaire (SoyFFQ) was used to investigate the soybean intake of 469 women in different communities in Nanjing. Based on the data of the American food composition, two soy isoflavones (genistein and daidzin Yuan daidzein daily intake; 30 subjects were provided urine samples, the concentration of soy isoflavone metabolites in urine was determined by UPLC-MS / MS; Determination of genistein and daidzein in some soy foods by liquid chromatography (HPLC). [Results] Estimated dietary intakes of 469 women: genistein: (50.7 ± 31.6) mg / d, daidzein: (33.4 ± 35.9) mg / d; urine Determination of soy isoflavone metabolites: genistein :( 515.9 ± 674.5) μmol / mol, daidzein: (927.7 ± 1107.2) μmol / mol. The correlation coefficients between dietary intakes of genistein and daidzein and those of urine soy isoflavone metabolites were 0.59 and 0.57, respectively (P <0.05). [Conclusion] It is considered that the estimates of genistein and daidzein intake correlate with the urine test values and provide reliable data for the related studies on the risk of developing soy isoflavones and breast cancer in China And method.