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通过对聚(γ-苄基L-谷氨酸酯)(PBLG)的亲水改性制备了两亲性聚(γ-苄基L-谷氨酸酯-co-羟乙谷酰胺)无规共聚肽(PBHG)用于静电纺丝制备超细纤维.通过傅里叶变换红外光谱、核磁共振氢谱表征了聚合物结构.通过测定溶液表面张力、黏度、电导率及扫描电镜观察纤维形貌考察了不同溶剂及PBHG浓度对纺丝溶液性质及电纺纤维的影响.通过水浸实验及MTT法评价了电纺纤维膜的亲水性及细胞相容性.研究发现在三氯甲烷(TCM)和四氢呋喃(THF)中PBHG采取α-螺旋构象,刚性分子链自取向排列,可获得直径为微米或亚微米的电纺纤维.以TCM为溶剂时,因溶液表面张力大、导电率低导致纤维品质较差,而以THF为溶剂可获得表面光洁、尺寸均匀的电纺纤维.当溶剂为三氟乙酸(TFA)时,PBHG采取无规线团构象,柔性分子链彼此缠结,同时溶液表面张力小、黏度低、电导率高,可获得纳米电纺纤维.但因TFA挥发性相对较差,易造成纤维粘连.将TFA与TCM复配后作为溶剂可改善纤维粘连问题.与PBLG电纺纤维相比,改性后的PBHG电纺纤维的亲水性得到了改善,可在水中保持纤维骨架而无需交联,并表现出良好的细胞相容性,能促进细胞在电纺纤维膜上的增殖.
Amphiphilic poly (γ-benzyl L-glutamate-co-hydroxybutylamide) random was prepared by hydrophilic modification of poly (γ-benzyl L-glutamate) The copolymer peptide (PBHG) was used in electrospinning to prepare ultrafine fibers.The structure of the polymer was characterized by Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy.The surface tension, viscosity, conductivity and scanning electron microscopy The effects of different solvents and PBHG concentration on spinning solution properties and electrospun fibers were investigated.The hydrophilicity and cytocompatibility of the electrospun fibrous membranes were evaluated by water immersion test and MTT method.The results showed that in the presence of trichloromethane (TCM) ) And THF (THF), the α-helical conformation of PBHG was taken, and the rigid molecular chains were self-aligned to obtain electrospun fibers with a diameter of micrometer or submicron.When TCM was used as a solvent, due to the large surface tension of the solution and low conductivity Fibers with poor fiber quality and smooth surface and uniform size of electrospun fibers with THF as solvents.When the solvent is trifluoroacetic acid (TFA), PBHG adopts random coil conformation and the flexible molecular chains are intertwined with each other, while the solution Small surface tension, low viscosity, high conductivity, can be obtained nanofiber TFA volatility is relatively poor, easy to cause fiber adhesions.TFA and TCM compound as a solvent can improve the fiber adhesion problems.Compared with PBLG electrospun fibers, modified PBHG electrospun fibers hydrophilicity is improved , Can maintain the fiber skeleton in the water without the need for cross-linking, and shows good cell compatibility, can promote cell proliferation in the electrospun fiber membrane.