论文部分内容阅读
应用改性甲醇—水为流动相,在YWG—C_(18)H_(37)反相柱上,以异搏定(verapamil)为内标,建立了适合于临床生化研究和药物代谢动力学研究的同时或分别测定人血浆中利多卡因(lidocaine),布比卡因(bupivacaine)和丁卡因(tetracaine)的高效液相色谱法。本法简便、快速、灵敏、重现性好,流动相可循环使用。利多卡因、布比卡因和丁卡因的平均回收率分别为101±3.6%,89.3±4.1%和80±10.1%,不同浓度水平测定结果的日内和日间变异系数一般小于10%,其检定限度(信噪比>3)分别为0.03,0.05和0.1μg/ml。
On the YWG-C 18 H 37 column with modified methanol-water as mobile phase, verapamil was used as an internal standard to establish a suitable biochemical and pharmacokinetic study Simultaneous or separate determination of lidocaine, bupivacaine and tetracaine in human plasma by high performance liquid chromatography. This method is simple, fast, sensitive, reproducible, mobile phase can be recycled. The average recoveries of lidocaine, bupivacaine and tetracaine were 101 ± 3.6%, 89.3 ± 4.1% and 80 ± 10.1%, respectively. The intra-and inter-day variation coefficients of different concentration levels were generally less than 10% The test limit (signal to noise ratio> 3) were 0.03,0.05 and 0.1μg / ml.