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采用基因分段克隆、表达结合蛋白质印迹 ,筛选到了口蹄疫病毒非结构蛋白 3ABC上高结合力、保守的感染相关线性表位 ,分别位于 3ABC蛋白上第 10 6～ 15 5和 15 6～ 190位氨基酸 .这两个表位可与感染不同血清型口蹄疫病毒动物康复血清反应 ,但不与来自健康免疫动物和未接触病毒动物的血清发生反应 .实验表明 ,用基因工程表达的多肽筛选抗原表位的方法是可行的 .
Using segregation of genes and expression of binding proteins, high-binding and conservative infection-associated linear epitopes of 3ABC on non-structural protein 3ABC of foot-and-mouth disease virus were screened, which were respectively located at amino acids 10 6-15 5 and 15 6-1 190 of 3ABC protein These two epitopes can react with the serum of animals infected with different serotypes of foot-and-mouth disease virus but not with the serum from both healthy and non-virus-infected animals.Experiments have shown that screening of antigenic epitopes using genetically engineered polypeptides The method is feasible.