论文部分内容阅读
目的探讨肿瘤坏死因子相关的凋亡诱导配体(TRAIL)联合相关小分子药物对胰腺癌细胞的抑制作用。方法运用MTT、流式细胞仪和Western blot方法观察TRAIL、白藜芦醇、紫杉醇和三氧化二砷(或药物组合)对人不同胰腺癌细胞系的敏感性及协同效应。结果单独使用TRAIL、白藜芦醇或紫杉醇没有引起capan-2细胞明显的凋亡。将TRAIL和白藜芦醇或紫杉醇联合使用,均引起capan-2细胞的明显凋亡;其对colo-357细胞也有很强的协同作用。几种药物对Miapaca-2细胞都有较明显抑制作用;但在capan-1细胞中,几种药物基本无协同作用。结论 TRAIL与白藜芦醇或紫杉醇联用可以提高TRAIL对胰腺癌细胞的凋亡诱导效果。
Objective To investigate the inhibitory effect of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) combined with related small molecule drugs on pancreatic cancer cells. Methods The sensitivity and synergistic effect of TRAIL, resveratrol, paclitaxel and arsenic trioxide (or drug combination) on human pancreatic cancer cell lines were observed by MTT, flow cytometry and Western blot. Results The use of TRAIL, resveratrol or paclitaxel alone did not cause significant apoptosis in capan-2 cells. The combination of TRAIL with resveratrol or paclitaxel caused significant apoptosis in capan-2 cells; it also had a strong synergistic effect on colo-357 cells. Several drugs have a significant inhibitory effect on Miapaca-2 cells; however, few drugs in capan-1 cells have little synergistic effect. Conclusion The combination of TRAIL with resveratrol or paclitaxel can increase the apoptosis-inducing effect of TRAIL on pancreatic cancer cells.