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微卫星标记已在人类与动物的遗传作图中得到了广泛应用.微卫星标记所揭示的是简单顺序长度多态性(Simple Sequence Length Polymorphism,SSLP),可利用PCR方法检测,因而快速简便,而且所需DNA量少.微卫星标记在遗传分析中表现为共显性,具有高度的多态性,往往有多个等位形式存在,信息含量很高,目前,在植物中微卫星标记主要用于基因型分析,品系分析及基因定位,尚未大规模用于遗传作图,在水稻中,通过筛选基因组文库和检索DNA序列库,已经获得了58个微卫星标记.本研究对一个水稻双单倍体(DH)群体进行了微卫星标记遗传作图工作,将89个微卫星标记较均匀地定位在已有的遗传图谱上,为今后应用微卫星标记定位水稻基因和标记辅助育种奠定了基础
Microsatellite markers have been widely used in human and animal genetic mapping.Microsatellite markers reveal Simple Sequence Length Polymorphism (SSLP), which can be detected by PCR method, so it is fast and easy, But also requires less DNA.Microsatellite markers appear to be co-dominant in genetic analysis, highly polymorphic, often exist in multiple allelic forms and have high information content. At present, microsatellite markers in plants are mainly For genotyping, lineage analysis and gene mapping, not yet used in large-scale genetic mapping, in the rice, 58 microsatellite markers have been obtained by screening genomic libraries and searching DNA sequence libraries.In this study, Homology (DH) populations were subjected to microsatellite marker genetic mapping, 89 microsatellite markers were more evenly located on the existing genetic map, laid the foundation for the future application of microsatellite markers to locate rice genes and marker-assisted breeding basis