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采用平板培养法和梯度凝胶电泳法,分别对不同植株密度苔藓(主要为齿肋赤藓,纯度>95%)结皮土壤中可培养、非培养微生物的数量和物种多样性进行了研究。结果表明:自然状态下细菌是苔藓结皮土壤中的优势微生物类群;随着苔藓植株密度降低可培养微生物中细菌比重下降而放线菌比重上升;可培养微生物的数量随着苔藓植株密度降低而下降,物种多样性无显著变化;非培养细菌的数量和物种数表现出与可培养微生物相反的变化趋势。初步推断造成两者差异的主要原因在于:1)研究方法不同,平板培养反映了细菌、放线菌和真菌三种不同微生物的数量和种类变化,而梯度凝胶电泳技术仅反映了细菌的数量和多样性变化;2)可培养微生物多为土壤中的优势种属,而梯度凝胶电泳技术则可以检测到更多的非优势物种。
The number and species diversity of culturable and non-cultured microorganisms in the soil with different plant density moss (mainly erythrosuria, purity> 95%) were studied by plate culture and gradient gel electrophoresis. The results showed that bacteria were the dominant microorganisms in moss crust soil under natural conditions. With the decrease of density of moss plants, the specific gravity of bacteria decreased and the proportion of actinomycetes increased. The number of culturable microorganisms decreased as the density of moss plants decreased Decreased, there was no significant change in species diversity; the number of non-cultured bacteria and the number of species showed the opposite trend of cultureable microorganisms. The main reasons for the difference between the two are as follows: 1) The research methods are different, and the plate culture reflects the changes of the number and species of three different microorganisms such as bacteria, actinomycetes and fungi. However, gradient gel electrophoresis only reflects the number of bacteria And diversity changes. 2) Most of the cultivable microorganisms are the dominant species in the soil, whereas gradient gel electrophoresis can detect more non-dominant species.