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目的:研究补肾益精代表方左归丸对衰老大鼠骨髓间充质干细胞(MSC)增殖的影响。方法:含D-半乳糖完全培养基诱导MSCs,制备衰老MSCs模型。观察各组MSCs形态,生长周期,细胞生长曲线,β-半乳糖苷酶衰老细胞染色结果。结果:模型组大鼠MSC OD值明显低于正常组,左归丸组大鼠MSC OD值明显高于模型组(P<0.05)。模型组衰老细胞阳性率高于正常组,左归丸组衰老细胞阳性率明显低于模型组(P<0.05)。结论:左归丸能提高衰老大鼠MSC减弱的增殖能力。
Objective: To study the effect of Zuogui pill, a representative of Bushen Yijing, on the proliferation of bone marrow mesenchymal stem cells (MSCs) of senile rats. Methods: MSCs were induced by D-galactose-containing complete medium to prepare aging MSCs model. The morphology, growth cycle, cell growth curve and the results of β-galactosidase senescence cells in each group were observed. Results: The OD value of MSC in model group was significantly lower than that in normal group. The OD value of MSC in Zuogui Pill group was significantly higher than that in model group (P <0.05). The positive rate of senescent cells in model group was higher than that in normal group. The positive rate of senescent cells in Zuoguiwan group was significantly lower than that in model group (P <0.05). Conclusion: Zuogui pill can improve the weakened proliferation ability of MSCs in aging rats.