心肌缺血后再灌注损伤是个重要问题,近十年的研究取得了成果,但也存在问题。成果的取得与数学、物理学及物理化学技术的引入有关。如氧自由基损伤作用肯定就是一个例证。1987年前,人们用化学方法说明再灌注时有氧自由基生成,或用已知自由基清除剂观察保护效果,间接证明氧自由基的参与。1987年Zweir等用顺磁共振(ESR)技术直接检测到心肌再灌注后出现的氧自由基信号。对于波谱的辨认,自由基归属及方法学等方面虽有争议,但由于顺磁捕集技术的引入,能直接看到各种短寿命氧自由加成物的信号及依次出现的过程,从而证实了缺血后再灌注过程中有大量氧自由基产生。此外,顺磁标记技术的应用,使生物膜损伤的研究深入一步,用不同链长的脂肪酸及蛋白巯基顺磁标记物分别插入大鼠心肌细胞质膜中,通过不同深度的膜脂流动性改变,蛋白质运动及构象改变,清楚地揭示了氧自由基对生物膜的损害。有的作者用分离的缺血心肌线粒体进行顺磁标记,发现肌膜 Myocardial ischemia and reperfusion injury is an important issue, the results of the past ten years of research, but there are also problems. The achievement of the results and the introduction of mathematics, physics and physical chemistry technology. Such as oxygen free radical damage is certainly an example. Before 1987, people used chemical methods to explain the generation of oxygen free radicals during reperfusion, or observe the protective effect with known free-radical scavengers, indirectly demonstrating the participation of oxygen free radicals. In 1987, Zweir et al. Directly detected the oxygen free radical signal after myocardial reperfusion with paramagnetic resonance (ESR) technique. Although there is controversy over spectrum identification, radical assignments, and methodological aspects, the introduction of paramagnetic capture technology can directly reveal the signals of various short-lived oxygen free adducts and the subsequent processes, thus confirming A large number of oxygen free radicals were produced during the reperfusion after ischemia. In addition, the application of paramagnetic marker technology makes the study of biofilm damage one step further. The fatty acid with different chain length and the protein sulfhydryl paramagnetic markers are respectively inserted into the plasma membrane of rat cardiomyocyte, and through the fluidity change of membrane lipid at different depths, Changes in protein motility and conformation clearly reveal the damage of biofilms by oxygen radicals. Some authors use isolated mitochondria of ischemic myocardium for paramagnetic labeling and find the sarcolemma