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为分析邢台地区致鸡卵黄性腹膜炎大肠杆菌(E.coli)分离株的血清型、毒力基因和耐药性,本实验采用常规鉴定方法和16S rRNA PCR的方法,从邢台地区不同养鸡场46份患卵黄性腹膜炎的40周龄~45周龄蛋鸡病料组织中分离鉴定出37株E.coli。人工感染1日龄的雏鸡,结果显示32株分离菌为致病性E.coli,采用玻板凝集法检测其血清型分布,结果显示定型的27株致病性E.coli分离株共属于11个血清型,以O_(78)、O_(148)、O_2及O_8为优势血清型。采用PCR方法检测32株致病性E.coli分离株14种毒力基因,其中irp2、fyuA、ompT、Iss~a、iucD~a、iutA、iroN、hlyF、fimC毒力基因检出率最高,分别为100%、96.9%、93.8%、86.7%、84.3%、71.9%、65.7%、56.3%和50%,其余毒力基因检出率较低。耐药性分析结果显示,32株致病性E.coli分离株仅对头孢克肟、左氧氟沙星、阿奇霉素、头孢曲松和氟苯尼考5种药物高度敏感,对其他药物存在不同程度的耐药性。本研究为防治致病性E.coli引起蛋鸡卵黄性腹膜炎提供了实验依据。
In order to analyze the serotypes, virulence genes and drug resistance of avian egg yolk peritonitis Escherichia coli isolates from Xingtai area, we used routine identification method and 16S rRNA PCR method to analyze the serotype, Forty-seven strains of E.coli were isolated and identified from 46 tissues of laying hens from 40 weeks to 45 weeks with vitelline peritonitis. Artificial infection of 1-day-old chicks, the results showed that 32 isolates of pathogenic E. coli, using the glass plate agglutination test serotype distribution, the results showed that the stereotypes of 27 pathogenic E. coli isolates belong to 11 Serotypes of serotypes were O_ (78), O_ (148), O_2 and O_8. The virulence genes of 32 strains of pathogenic E.coli were detected by PCR. Among them, the virulence genes of irp2, fyuA, ompT, Iss ~ a, iucD ~ a, iutA, iroN, hlyF and fimC were the highest, Which were 100%, 96.9%, 93.8%, 86.7%, 84.3%, 71.9%, 65.7%, 56.3% and 50% respectively. The detection rate of other virulence genes was lower. The results of drug resistance analysis showed that 32 strains of pathogenic E.coli isolates were highly sensitive to 5 kinds of drugs of cefixime, levofloxacin, azithromycin, ceftriaxone and florfenicol, and had different degrees of resistance to other drugs Sex. This study provides experimental evidence for the prevention and treatment of pathogenic E.coli egg yolk peritonitis in laying hens.