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将富集２，４－Ｄ的固氮根瘤菌接种到２～３叶期小麦根上，可诱发小麦形成类根瘤（Ｐａｒａｎｏｄｕｌｅｓ），经ＩＮＴ试剂检验和电镜切片观察，证明类根瘤中导入了固氮根疤菌。为２，４－Ｄ诱发结瘤技术提供了一种简比方法。
Inoculation of 2,4-D-rich rhizobia into 2 or 3 leaf stage wheat roots induces the formation of the type of root nodules (Paranodules) in wheat. The results of the INT reagent and electron microscopy showed that the root nodules were introduced with nitrogen-fixing scars bacteria. Provides a simpler method for 2,4-D induced nodulation.