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尾加压素Ⅱ受体(UrotensinⅡreceptor,UT)是一种7次跨膜G蛋白偶联受体,是目前发现的具有最强收缩血管作用的尾加压素Ⅱ的特异性受体。斑马鱼中存在4种不同的UT-like基因(uts2r1,uts2r2,uts2r3和uts2r4),分别位于斑马鱼4条不同染色体上。这4种uts2r基因核苷酸序列各不相同,uts2r1位于3号染色体上,其与12号染色体上的uts2r2基因蛋白序列相似度最高为47%,与6号染色体上的uts2r3和16号染色体上的uts2r4蛋白相似度分别是41.6%和33%。通过设计特异引物检测每种uts2r基因在斑马鱼成鱼各组织中的表达情况,结果显示3号染色体上的uts2r1基因在肾脏中表达量最高,6号染色体上的uts2r2基因在脊髓中表达量最高,12号染色体上的uts2r3基因在心脏中表达量最高,而16号染色体上的uts2r4基因在背肌中表达量最高。
Urotensin Ⅱ receptor (UT) is a seven-transmembrane G-protein coupled receptor that is the specific receptor of urotensin Ⅱ with the most vasoconstrictive effect. There are four different UT-like genes (uts2r1, uts2r2, uts2r3 and uts2r4) in zebrafish, which are located on four different chromosomes of zebrafish respectively. The four uts2r gene nucleotide sequences are different, uts2r1 is located on chromosome 3, which has a similarity of 47% with the uts2r2 gene sequence on chromosome 12, and on uts2r3 and chromosome 16 on chromosome 6 The uts2r4 protein similarity was 41.6% and 33%, respectively. By designing specific primers to detect the expression of each uts2r gene in various zebrafish adult tissues, the results showed that the uts2r1 gene on chromosome 3 was the highest in kidney and the uts2r2 gene on chromosome 6 was the highest in spinal cord , The uts2r3 gene on chromosome 12 was the highest in the heart, while the uts2r4 gene on chromosome 16 was the highest in the back muscle.