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以五味子品种“嫣红”为试材,采用植物效率仪Pocket-PEA研究了热胁迫处理对光系统活性的影响。结果表明:随着温度的升高,原初最大光化学效率(Fv/Fm)和叶片捕获的激子将电子传递到电子传递链中QA-下游电子受体的概率(ψ0)在35~40℃的范围内并没有明显变化,当温度升高到44℃和48℃时,则显著下降,ψ0的下降说明电子传递链受体侧受到了严重的抑制。随着温度升高,相对可变荧光在K点和J点的值(Vk和Vi)逐渐上升,但在44℃和48℃较对照呈显著上升。随着热胁迫时间的延长,Vk和Vi逐渐升高,胁迫5min后在K点和J点呈现双峰曲线,随胁迫时间延长Vk虽逐渐升高,但K点的峰逐渐消失,J点的峰则逐渐凸显。说明高温引起电子传递链供体侧和受体侧受到伤害,但供体侧对高温更加敏感。
Taking Schisandra chinensis variety “Yanhong ” as test material, the plant efficiency meter Pocket-PEA was used to study the effect of heat stress treatment on photosystem activity. The results showed that the initial maximum photochemical efficiency (Fv / Fm) and the probability of electron transfer to QA-downstream electron acceptor in the electron transport chain (ψ0) at 35-40 ℃ There was no significant change in the range of the electron transport chain. When the temperature was raised to 44 ℃ and 48 ℃, the decrease of ψ0 indicated that the acceptor side of the electron transport chain was severely inhibited. With the increase of temperature, the values of relative variable fluorescence at K and J (Vk and Vi) gradually increased, but increased significantly at 44 and 48 ℃. With the prolonging of heat stress, Vk and Vi gradually increased. After stress 5min, there were bimodal curves at K and J, and Vk increased gradually with the prolongation of stress but gradually disappeared at K and J The peak is gradually highlighted. High temperature caused by the electron transfer chain donor side and receptor side were harmed, but the donor side is more sensitive to high temperatures.