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[目的]研究富硒平菇对老年大鼠脾淋巴细胞的凋亡和血清sIL-2R分泌的影响。[方法]健康18月龄Wistar大鼠66只,雌雄各半,随机分为对照组,富硒平菇A、B组,亚硒酸钠A、B组,普通平菇A、B组。与基础饲料同时给予:含0.4mg/kg或0.6mg/kg含硒量的富硒平菇或亚硒酸钠或等重量普通平菇。123d末处死,测定全血硒含量,血浆sIL-2R含量。流式细胞术检测脾脏淋巴细胞的凋亡情况。[结果](1)饲料添加0.4mg/kg和0.6mg/kg富硒平菇能升高全血硒含量;抑制脾淋巴细胞凋亡,并有剂量依赖关系。两组抑制率分别为31.62%和40.70%;(2)0.4mg/kg富硒平菇使血清sIL-2R含量降低,0.6mg/kg富硒平菇使其升高;(3)与同剂量亚硒酸钠组比较,富硒平菇A、B组脾淋巴细胞凋亡抑制率更高、sIL-2R含量更低。[结论]富硒平菇可抑制老年大鼠脾淋巴细胞凋亡,适量补充可降低sIL-2R分泌,并调节老年大鼠免疫功能。
[Objective] To investigate the effects of Pleurotus ostreatus on the apoptosis of splenic lymphocytes and the secretion of serum sIL-2R in aged rats. [Methods] 66 healthy 18-month-old Wistar rats were randomly divided into control group, Se-enriched Pleurotus ostreatus group A and B, sodium selenite A and B groups and ordinary Pleurotus ostreatus group A and B. Simultaneous with basic feed: selenium-enriched Pleurotus ostreatus or sodium selenite containing 0.4 mg / kg or 0.6 mg / kg selenium, or equal weight normal mushroom. At the end of 123d death, the determination of whole blood selenium content, plasma sIL-2R content. Flow cytometry was used to detect apoptosis of spleen lymphocytes. [Results] (1) Feed supplementation with 0.4 mg / kg and 0.6 mg / kg Se-enriched Pleurotus eryngii increased selenium content in whole blood, inhibited splenic lymphocyte apoptosis in a dose-dependent manner. The inhibitory rates of two groups were 31.62% and 40.70% respectively. (2) 0.4mg / kg selenium-enriched Pleurotus eryngii reduced serum sIL-2R and 0.6mg / kg Se-enriched Pleurotus ostreatus. (3) Compared with sodium selenite group, the inhibitory rates of apoptosis of spleen lymphocytes of Se-enriched Pleurotus ostreatus group A and B were higher, and the content of sIL-2R was lower. [Conclusion] Se-enriched Pleurotus ostreatus can inhibit the apoptosis of splenic lymphocytes in aged rats. The moderate supplementation can reduce the secretion of sIL-2R and regulate the immune function in aged rats.