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分离和研究疟疾感染蚊的差异表达基因 ,对阐明媒介与疟原虫之间相互作用及其分子机制尤为重要。利用已建立的斯氏按蚊感染约氏疟原虫的差减cDNA库的进行表达筛选 ,发现表达增高基因中有一个编码与黑腹果蝇泛素羧端水解酶高度同源蛋白的序列。相似性比较显示该编码序列在氨基酸水平与已知的冈比亚按蚊EST序列对应部位的同源性为 89% ,与果蝇和人类的同源性均为 63%。模拟Northern印迹的表达动态分析提示 ,感染后至少 1~ 7天内该基因在蚊体内的表达显著增高 ,与疟原虫发育动合子穿越蚊中肠壁和子孢子从卵囊向蚊眼涎腺移行等关键阶段相一致。目前对有关蚊天然免疫系统激活的泛素途径所知甚少 ,现有结果提示该基因与疟原虫感染相关 ,它的克隆和表达分析有可能推测其在疟原虫感染中所起的作用
Isolating and studying differentially expressed genes of malaria-infected mosquitoes is of particular importance in elucidating the interaction between malaria vectors and their molecular mechanisms. Screening for the established subtracted cDNA library of Anopheles stephensi infected with Plasmodium yoelii showed that there was a sequence encoding a highly homologous protein with the Drosophila urocapsid carboxy terminal hydrolase in the increased expression gene. The similarity comparison showed that the homology of the coding sequence with that of the known Anopheles gambiae EST sequence was 89% at amino acid level, and 63% with Drosophila and human. Dynamic analysis of the expression of simulated Northern blots suggested that the expression of this gene in mosquitoes was significantly increased at least 1 to 7 days after infection and was associated with the development of malaria parasite through the midgut wall and sporozoites from the oocysts to the salivary glands in the mosquito The same phase. At present, little is known about the ubiquitin pathway that activates the innate immune system in mosquitoes. Current results suggest that the gene is associated with Plasmodium infection and its cloning and expression analysis may predict its role in Plasmodium infection