论文部分内容阅读
目的 探讨雷帕霉素对多发性骨髓瘤(MM)细胞株RPMI8226和U266凋亡的影响.方法 应用雷帕霉素250nmmol/L处理RPMI8226和U266细胞,常规HE染色观察细胞形态学变化,流式细胞术检测细胞凋亡情况,实时定量PCR和Western blot法分别检测细胞凋亡相关因子Bcl-2、Bax、Caspase-3、Caspase-8 mRNA和蛋白表达.其结果与不用雷帕霉素处理的空白对照组细胞比较.结果 与空白对照组比较,雷帕霉素组细胞核出现固缩及碎裂,凋亡小体的数量增多,RPMI8226和U266细胞凋亡率增加[(25.36±0.68)％ vs.(4.43±0.30)％ 和(22.72±2.61)％ vs.(5.66±0.80)％](P<0.05),Bax、Caspase-3、Caspase-8的mRNA和蛋白表达量增加,Bcl-2 mRNA和蛋白表达量减少(P<0.05).结论 雷帕霉素可能通过Caspases途径促进MM细胞株RPMI8226和U266凋亡.“,”Objective To investigate the effects of rapamycin on cell apoptosis of multiple myeloma(MM) cell lines RPMI8226 and U266.Methods MM cell lines RPMI8226 and U266 were treated with rapamycin 250 nmmol/L.HE staining was used to observe the morphological changes of apoptosis under optical microscope.Cell apoptosis was evaluated by flow cytometry.The expressions of Bcl-2,Bax,Caspase-3 and Caspase-8 were detected by real-time quantitative PCR and Western blot.The results were compared to those in the cells without rapamycin treatment.Results Compared to the cells without rapamycin treatment,the nucleus pyknosis and fracture occurred in rapamycin-treated cells with increased number of apoptotic body.The cell apoptosis rates of RPMI8226 and U266 were higher in rapamycin-treated cells than those in the cells without[(25.36±0.68)％ vs.(4.43±0.30)％ and (22.72±2.61)％ vs.(5.66±0.80)％](P<0.05).The mRNA and protein expressions of Bax,Caspase-3 and Caspase-8 were increased while Bcl-2 mRNA and protein expressions were decreased in rapamycin-treated cells as well(P<0.05).Conclusion Rapamycin can promote cell apoptosis of MM cell lines RPMI8226 and U266,which may be induced through Caspases signaling pathway.