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同济医科大学附属协和医院黄土昂博士在美国Becton Dickinson公司单克隆抗体研究中心从事造血细胞筛选及调控的博士后研究期间,利用三色兔疫荧光流式细胞术,分离出4种CD34阳性细胞亚群。作者分别进行单个细胞培养,发现CD34+/HLA-DR-/CD38-在形态学上含有原始的间质细胞和原始的造血细胞。后来通过一系列的接种和传代培养实验,应用光学/电子显微镜检查和组织化学及免疫组织分析,证实在骨髓存在一种能够序列分化成为造
At the post-doctoral study of hematopoietic cell selection and regulation by the Becton Dickinson Monoclonal Antibody Research Center in the United States, Dr. Huang Tuo-ang, affiliated Union Hospital of Tongji Medical University, used three-color rabbit immunofluorescence flow cytometry to isolate four CD34 positive cell subpopulations . The authors performed a single cell culture separately and found that CD34 + / HLA-DR- / CD38- morphologically contains primitive mesenchymal cells and primitive hematopoietic cells. Later through a series of inoculation and subculture experiments, using optical / electron microscopy and histochemical and immunohistochemical analysis confirmed that there is a bone marrow can differentiate into a series of