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目的:通过研究乳宁Ⅱ号方对小鼠Ca761乳腺癌移植瘤细胞周期及p53、ras表达的影响,探讨乳宁Ⅱ号方抑制乳腺癌生长的机制。方法:建立小鼠Ca761 乳腺癌移植模型,小鼠造模后随机分成生理盐水对照组、环磷酰胺(cyclophosphamide, CTX)治疗组、乳宁Ⅱ号方治疗组及乳宁Ⅱ号方加CTX 治疗组,每组12只。用流式细胞术检测经不同方法处理的荷瘤小鼠移植瘤细胞的细胞周期,同时运用免疫组化法测定移植瘤组织中p53和ras癌基因蛋白的表达,并进行组间比较。结果:乳宁Ⅱ号方治疗组、CTX治疗组与乳宁Ⅱ号方加CTX治疗组S期肿瘤细胞与生理盐水对照组比较明显减少(P<0.05);乳宁Ⅱ号方治疗组G0 G1期肿瘤细胞百分比低于CTX治疗组(P<0.05),而G2 M期细胞百分比高于CTX治疗组(P<0.05);乳宁Ⅱ号方治疗组与乳宁Ⅱ号方加CTX治疗组p53的表达均显著低于生理盐水对照组(P<0.05),而CTX对p53表达的影响不显著(P>0.05);乳宁Ⅱ号方治疗组、CTX治疗组与乳宁Ⅱ号方加CTX治疗组ras的表达均低于生理盐水对照组(P<0.05)。结论:乳宁Ⅱ号方可通过调控小鼠Ca761 乳腺癌移植瘤细胞的细胞周期来影响肿瘤的生长,这一作用与其调控肿瘤组织p53和ras基因表达有关。
OBJECTIVE: To investigate the effect of Runing Recipe No.2 on the cell cycle and the expression of p53 and ras in Ca761 breast cancer xenografts in mice and to explore the mechanism of Runing Recipe Ⅱ inhibiting the growth of breast cancer. Methods: The mouse model of Ca761 breast cancer was established. The mice were randomly divided into normal saline control group, cyclophosphamide (CTX) treatment group, Runing Ⅱ treatment group and Runing Ⅱ plus CTX treatment Group, 12 in each group. Flow cytometry was used to detect the cell cycle of transplanted tumor cells of tumor-bearing mice treated by different methods. At the same time, the expression of p53 and ras oncogene protein in the transplanted tumor tissue was detected by immunohistochemistry and compared between groups. Results: Compared with the control group, the number of tumor cells in Runing Ⅱ treatment group, CTX treatment group and Runing Ⅱ plus CTX treatment group were significantly decreased (P <0.05) (P <0.05), while the percentage of cells in G2 M phase was higher than that in CTX treatment group (P <0.05). The expression of p53 (P <0.05). However, the effect of CTX on p53 expression was not significant (P> 0.05). The expression of p53 The expression of ras in the treatment group was lower than that in the saline control group (P <0.05). Conclusion: Runing Recipe Ⅱ can affect the tumor growth by regulating the cell cycle of mouse Ca761 breast cancer xenografts, which is related to the regulation of p53 and ras gene expression in tumor tissues.