内毒素在革兰氏阴性杆菌感染引起休克的发生发展过程中起重要作用,而肺组织的瘀血、水肿并发展为呼吸窘迫综合征是休克病人难以救治的重要原因。因此,内毒素对肺血管内皮的损伤及产生前列环素的影响是一个值得研究的问题,本实验用培养的牛肺动脉内皮细胞进行,24孔板内每孔细胞数约2×10~5个,每孔加铬酸钠~(51)Cr 2μCi,37℃孵育40分钟,弃上清,冲洗后加入含内毒素50μg/ml的培养基,37℃孵育30分钟,取上清在γ计 Endotoxin plays an important role in the development of shock caused by Gram-negative bacilli infection. However, the stasis and edema of lung tissue and the development of respiratory distress syndrome are the important reasons for the difficulty in treating patients with shock. Therefore, endotoxin on the pulmonary vascular endothelial damage and the impact of prostacyclin production is a worthy of study, the experiment with cultured bovine pulmonary artery endothelial cells, the number of cells per well in a 24-well plate about 2 × 10 ~ 5 , Each well with sodium chromate ~ (51) Cr 2μCi, incubated at 37 ℃ for 40 minutes, the supernatant was discarded, rinsed with 50μg / ml endotoxin medium, incubated for 30 minutes at 37 ℃, the supernatant in γ