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Interleukin 12(IL-12)and/or interleukin 18(IL-18)gene ablated mice were applied for the investigation of thetissue expression of interferon γ(IFN-γ).For IL-12~(-/-),IL-18~(-/-),IL-12~(-/-)/18~(-/-) and wt mice,reproductive performancewere recorded and IFN-γ concentrations in heart,lung,liver,spleen,kidney and serum were quantified by ELISA.There were no significant differences of IFN-γ in heart,lung and kidney between 4 strains although control groupwas higher.It was observed that for IL-12~(-/-) mice,compared with other 3 groups,IFN-γ in liver and spleen weredecreased(p<0.05)and reproductive performance appeared to be impaired.Serum IFN-γ level of IL-12~(-/-)/18~(-/-)mice was significantly higher(p<0.05).It was showed that IFN-γ productions under the normal condition wereindependent upon IL-12 and IL-18,its expressions in various tissues were different,and optimal IFN-γ is necessaryfor the normal growth and development of mammals.This study is helpful for clinical cytokines therapy.Cellular& Molecular Immunology.2005;2(1):68-72.
Interleukin 12 (IL-12) and / or interleukin 18 (IL-18) gene ablated mice were applied for the investigation of the tissue expression of interferon γ (IFN-γ) IL-12 ~ (- / -) / 18 ~ (- / -) and wt mice, reproductive performancewere recorded and IFN-γ concentrations in heart, lung, liver, spleen, kidney and serum were quantified by ELISA. There were no significant differences of IFN-γ in heart, lung and kidney between 4 and control groups was higher. It was observed that for IL-12 ~ (- / -) mice, compared with other 3 groups, IFN -γ in liver and spleen were decreased (p <0.05) and reproductive performance was significantly increased (p <0.05) .Serum IFN-γ level of IL-12 ~ 0.05) .It was showed that IFN-γ productions under the normal condition were in dependent upon IL-12 and IL-18, its expressions in various tissues were different, and optimal IFN-γ is necessary for the normal growth and development of mammals .This study is helpful for clinical cytokines th erapy. Cellular & Molecular Immunology. 2005; 2 (1): 68-72.