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目的 :探讨外周血单个核细胞 (PBMC)Th1 、Th2 类细胞因子的表达与食管鳞状细胞癌临床病理因素之间的相关性。方法 :采用双抗体酶联免疫吸附法 (ELISA)检测 39例食管鳞状细胞癌患者和 2 0例健康对照者外周血单个核细胞Th1 (IL 2、INF γ)、Th2 (IL 4、IL 6、IL 1 0 )类细胞因子的含量水平 ,并与食管鳞状细胞癌病理因素之间进行相关分析。结果 :食管鳞状细胞癌患者PBMC中Th1 类细胞因子IL 2、INF γ的表达明显低于健康对照组 (P <0 .0 5) ,Th2 类细胞因子IL 6的表达明显高于对照组 (P <0 .0 5) ,存在Th1 /Th2 漂移现象 ;且发现TNM分期越晚 ,Th1 类细胞因子IL 2的表达越弱 ,而IL 6表达越强 ,存在显著性差异 (P <0 .0 5)。Th1 /Th2 漂移现象与肿瘤分化程度无明显相关性。结论 :食管鳞状细胞癌患者PBMC存在某些Th2 类细胞因子表达优势 ,而Th1 类细胞因子表达减弱 ;Th1 /Th2 漂移现象与肿瘤的TNM分期有关
Objective: To investigate the correlation between the expression of Th1 and Th2 cytokines in peripheral blood mononuclear cells (PBMC) and the clinicopathological factors of esophageal squamous cell carcinoma. Methods: The levels of Th1 (IL 2, INF γ), Th2 (IL 4, IL 6) in peripheral blood mononuclear cells of 39 patients with esophageal squamous cell carcinoma and 20 healthy controls were detected by enzyme linked immunosorbent assay (ELISA) , IL 1 0) cytokines levels, and with the correlation between esophageal squamous cell carcinoma pathological factors. Results: The expression of Th1 cytokines IL 2 and INF γ in PBMC from patients with esophageal squamous cell carcinoma was significantly lower than that from healthy controls (P <0.05), and the expression of Th2 cytokines IL 6 was significantly higher than that from the control group P <0.05), there was a Th1 / Th2 shift phenomenon. The later the TNM staging, the weaker the expression of Th1 cytokine IL 2, while the stronger the IL 6 expression, there was a significant difference (P <0. 5). There was no significant correlation between Th1 / Th2 shift and tumor differentiation. CONCLUSIONS: Some Th2 cytokines are expressed in PBMC of esophageal squamous cell carcinoma patients, but the expression of Th1 cytokines is weakened. The Th1 / Th2 shift is related to the TNM staging