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利用从大宝山尾矿库酸性矿山废水培养得到的氧化亚铁硫杆菌(A.f.菌)和闪锌矿进行初始pH2.00和pH3.00条件下的无菌氧化、含菌含铁氧化和含菌不含铁氧化实验。通过测定实验过程溶液的阳离子、硫酸根离子含量和pH值,实验固体产物的XRD分析和反应前后闪锌矿表面的电镜扫描(SEM)对比分析,获得如下结论:①在不含铁离子的情况下,闪锌矿在pH为2.00的环境下比pH为3.00的环境下更容易被氧化;②氧化亚铁硫杆菌对闪锌矿的氧化有明显的促进作用,其氧化速率比无菌氧化速率要提高10～30倍;③铁离子存在对闪锌矿生物氧化有明显的促进作用,最高可达300倍;④氧化亚铁硫杆菌以及铁离子存在使闪锌矿的生物氧化作用最终溶液pH值明显低于无菌氧化作用的pH值;⑤闪锌矿的微生物氧化的最佳pH条件在2.0～2.4之间。闪锌矿的生物氧化过程中,细菌的直接氧化作用和间接氧化作用可能都是存在的。
Aseptic oxidation, bacteria-containing ferrous oxidation and bacteria-containing bacteria were carried out under the initial pH of 2.00 and at pH 3.00 using the bacteria A. ferrooxidans (A. fumigatus) and sphalerite obtained from acid mine waste water from the tailing pond of Dabashan. Ferrous oxidation experiment. The results of X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis of the surface of the sphalerite before and after the determination of the cation, the content of the sulfate ion and the pH value of the solution in the experiment process are as follows: ① In the case of no iron ion , Sphalerite is more easily oxidized under the environment of pH 2.00 than pH 3.00; ②Tiobacillus ferrooxidans can obviously promote the oxidation of sphalerite, its oxidation rate is higher than that of the aseptic oxidation rate To be increased by 10 to 30 times; ③ iron ions have a significant role in promoting the biooxidation of sphalerite, up to 300 times; ④ Thiobacillus ferrooxidans and iron ions make the biological oxidation of sphalerite final solution pH Value was significantly lower than the aseptic oxidation pH; ⑤ microbial oxidation of sphalerite pH optimum conditions between 2.0 to 2.4. Sphalerite biooxidation process, bacterial direct oxidation and indirect oxidation may exist.