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目的:通过HBV前S/S区基因变异研究探讨HBV宫内感染的机制。方法:将HBsAg阳性母亲按照随访其新生儿是否发生宫内感染,分为病例组和对照组,经PCR扩增HBV DNA,基因克隆、测序,构建种系进化树分析前S/S基因的突变。结果:共得到60株HBV序列,来源于同一组的序列较为集中在树中的某枝,而且进化距离从病例组母亲、病例组患儿到对照组母亲存在由近及远的特征。结论:各组病毒株在基因序列进化上存在差异,某些突变位点在非宫内感染组母亲中的发生率较高,突变的发生可能使其所在的编码区功能发生变化而影响宫内感染的发生。
Objective: To explore the mechanism of HBV intrauterine infection through the study of gene mutation in pre-S / S region of HBV. Methods: HBsAg-positive mothers were divided into two groups according to whether their newborns were intrauterine infection at follow-up. HBV DNA was amplified by PCR, cloned and sequenced. The S / S gene mutation . Results: A total of 60 HBV sequences were obtained. The sequences from the same group were more concentrated in some branches of the tree, and the evolutionary distance was near and far from the mothers in the case group, the children in the case group to the mother in the control group. Conclusion: There are differences in gene sequence evolution of each group of virus strains, some of the mutation sites in the non-intrauterine infection group of mothers in the high incidence of mutations may affect the function of the coding region where it affects the intrauterine The incidence of infection.