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目的:探讨2000μW/cm2电磁辐射对大鼠海马N-甲基-D-门冬氨酸受体亚单位2A(NR2A)蛋白及其mRNA水平表达的影响,揭示电磁辐射对大鼠学习记忆功能的损伤机制。方法:实验分为空白对照组,假辐射组,1h/d、2h/d、3h/d辐射组。将辐射组大鼠固定体位,头部接受功率密度为2000μW/cm2的近场辐射,连续辐射30d。通过Morris水迷宫检测大鼠的空间学习记忆能力,采用免疫组化法和Western blot法检测大鼠海马组织NR2A蛋白表达的变化,RT-PCR法检测大鼠海马组织NR2A mRNA表达的变化。结果:假辐射组大鼠各项指标与空白对照组相比均无统计学差异(P>0.05);而各辐射组大鼠寻找安全平台的逃避潜伏期较空白对照组明显延长(P<0.05);各辐射组大鼠海马神经元排列紊乱,NR2A阳性细胞比率显著下降,海马组织NR2A蛋白及其mRNA表达水平明显降低(P<0.05)。结论:2000μW/cm2电磁辐射可导致大鼠学习记忆功能下降,其机制可能与大鼠海马组织NR2A蛋白及其mRNA的表达降低有关。
Objective: To investigate the effect of electromagnetic radiation at 2000μW / cm2 on the expression of NR2A protein and its mRNA in the hippocampus of rats, and to reveal the effect of electromagnetic radiation on learning and memory in rats Damage mechanism. Methods: The experiment was divided into blank control group, sham radiation group, 1h / d, 2h / d, 3h / d radiation group. Radiation rats were fixed position, the head to receive power density of 2000μW / cm2 near-field radiation, continuous irradiation 30d. The spatial learning and memory abilities of rats were detected by Morris water maze. The expression of NR2A protein in hippocampus was detected by immunohistochemistry and Western blot. The expression of NR2A mRNA in hippocampus was detected by RT-PCR. Results: There was no significant difference in all indexes between sham radiation group and blank control group (P> 0.05), while the escape latency of searching for safe platform in each radiation group was significantly longer than that of blank control group (P <0.05) The neurons in hippocampus were disordered and the ratio of NR2A positive cells was significantly decreased in hippocampus. The expression of NR2A protein and mRNA in hippocampus were significantly decreased (P <0.05). CONCLUSION: Electromagnetic radiation at 2000μW / cm2 can decrease the learning and memory ability of rats, which may be related to the decreased expression of NR2A protein and its mRNA in the hippocampus of rats.