论文部分内容阅读
为构建编码HBV表面抗原蛋白S的重组真核表达质粒pCR3.1-S,将它直接肌肉注射BAIB/c小鼠,以ELISA法检测免疫小鼠血清,另用~3H-TdR掺入法测定淋巴细胞增殖活性。结果表明,HBV基因疫苗pCR3.1-S可诱发小鼠产生体液和细胞免疫应答。
To construct recombinant eukaryotic expression plasmid pCR3.1-S encoding HBV surface antigen protein S, it was injected intramuscularly into BAIB / c mice and the serum of mice was detected by ELISA. The 3H-TdR incorporation assay Lymphocyte proliferation activity. The results showed that the HBV gene vaccine pCR3.1-S can induce humoral and cellular immune responses in mice.