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目的:检测在8种骨肉瘤细胞系中Runx3基因启动子区域甲基化状态和Runx2基因及Runx3基因的表达情况,探讨Runx2基因和Runx3基因对骨肉瘤形成的影响。方法:采用甲基化特异PCR(MSP)对8种骨肉瘤细胞系中Runx3基因启动子区域甲基化状态进行分析,采用逆转录多聚合酶链反应(RT-PCR)检测Runx3及Runx2基因的表达情况。结果:MSP结果显示,Runx3基因启动子区域无明显过甲基化状态发生,RT-PCR结果表明,大多数骨肉瘤细胞系中Runx2基因和Runx3基因均有不同程度表达。结论:在骨肉瘤细胞系中无明显的Runx3基因特异性表达异常,Runx3基因启动子区域未见CpG岛发生广泛甲基化等表观遗传学异常改变,提示Runx3基因与骨肉瘤的发生发展无必要联系。Runx2基因虽然在成骨细胞分化、软骨细胞成熟及骨基质蛋白产生中发挥重要作用,但在骨肉瘤细胞系中均有不同程度的表达。
OBJECTIVE: To detect the methylation status of Runx3 promoter and the expression of Runx2 gene and Runx3 gene in 8 osteosarcoma cell lines and to explore the effect of Runx2 gene and Runx3 gene on the formation of osteosarcoma. Methods: The methylation status of Runx3 promoter region in 8 osteosarcoma cell lines was analyzed by methylation-specific PCR (MSP). The expressions of Runx3 and Runx2 genes were detected by reverse transcription-polymerase chain reaction (RT-PCR) Express the situation. Results: The results of MSP showed that there was no obvious hypermethylation in the promoter region of Runx3 gene. The results of RT-PCR indicated that the expression of Runx2 gene and Runx3 gene were different in most osteosarcoma cell lines. CONCLUSIONS: There is no obvious Runx3 gene-specific expression in osteosarcoma cell lines, no apparent genetic abnormalities such as extensive methylation of CpG island in Runx3 promoter region suggest that Runx3 gene and osteosarcoma occur Necessary to contact. Although Runx2 gene plays an important role in osteoblast differentiation, chondrocyte maturation and bone matrix protein production, it is expressed in osteosarcoma cell lines to varying degrees.